Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.

Journal Article (Journal Article)

A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.

Full Text

Duke Authors

Cited Authors

  • St Clair, EW; Pisetsky, DS; Reich, CF; Chambers, JC; Keene, JD

Published Date

  • April 1, 1988

Published In

Volume / Issue

  • 31 / 4

Start / End Page

  • 506 - 514

PubMed ID

  • 3128988

International Standard Serial Number (ISSN)

  • 0004-3591

Digital Object Identifier (DOI)

  • 10.1002/art.1780310407

Language

  • eng

Conference Location

  • United States