Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.
Journal Article (Journal Article)
A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.
Full Text
Duke Authors
Cited Authors
- St Clair, EW; Pisetsky, DS; Reich, CF; Chambers, JC; Keene, JD
Published Date
- April 1, 1988
Published In
Volume / Issue
- 31 / 4
Start / End Page
- 506 - 514
PubMed ID
- 3128988
International Standard Serial Number (ISSN)
- 0004-3591
Digital Object Identifier (DOI)
- 10.1002/art.1780310407
Language
- eng
Conference Location
- United States