Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice.

Journal Article

The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Caster, SA; Piper, M; Scott, DW; Steinberg, AD

Published Date

  • March 1, 1984

Published In

Volume / Issue

  • 84 / 1

Start / End Page

  • 32 - 40

PubMed ID

  • 6607779

International Standard Serial Number (ISSN)

  • 0008-8749

Language

  • eng

Conference Location

  • Netherlands