In vitro autoantibody production by normal adult and cord blood B cells.

Published

Journal Article

To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimulated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Jelinek, DF; McAnally, LM; Reich, CF; Lipsky, PE

Published Date

  • March 1, 1990

Published In

Volume / Issue

  • 85 / 3

Start / End Page

  • 899 - 903

PubMed ID

  • 2138166

Pubmed Central ID

  • 2138166

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/JCI114517

Language

  • eng

Conference Location

  • United States