Inhibition of in vitro anti-DNA B-cell responses by cyclosporine.

Published

Journal Article

The action of the immunosuppressive agent cyclosporine (CsA) on anti-DNA B-cell responses was investigated in an in vitro system. Spleen cells from autoimmune MRL-lpr/lpr or control BALB/c mice, when cultured at high cell density, spontaneously produced significant amounts of IgM and IgG, including anti-DNA. IgG levels, both total and anti-DNA, were much higher for MRL-lpr/lpr cells compared to BALB/c cells, suggesting similarity of this model system to the in vivo response. For cells of both strains, the production of IgM and IgG anti-DNA was 10- to 100-fold more sensitive to the inhibitory activity of CsA than total immunoglobulin production. The effect was not manifest, however, in cultures stimulated with the B-cell mitogen, lipopolysaccharide. These observations suggest that CsA in certain dose ranges preferentially inhibits anti-DNA production, with efficacy determined by the mechanisms promoting B-cell hyperactivity.

Full Text

Duke Authors

Cited Authors

  • Mayus, JL; Semper, KF; Pisetsky, DS

Published Date

  • August 1985

Published In

Volume / Issue

  • 94 / 1

Start / End Page

  • 195 - 204

PubMed ID

  • 3874703

Pubmed Central ID

  • 3874703

International Standard Serial Number (ISSN)

  • 0008-8749

Digital Object Identifier (DOI)

  • 10.1016/0008-8749(85)90096-6

Language

  • eng

Conference Location

  • Netherlands