The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells.

Published

Journal Article

An understanding of replication initiation in mammalian cells has been hampered by the lack of mutations and/or inhibitors that arrest cells just prior to entry into the S period. The plant amino acid mimosine has recently been suggested to inhibit cells at a regulatory step in late G1. We have examined the effects of mimosine on cell cycle traverse in the mimosine [corrected]-resistant CHO cell line CHOC 400. When administered to cultures for 14 h after reversal of a G0 block, the drug appears to arrest the population at the G1/S boundary, and upon its removal cells enter the S phase in a synchronous wave. However, when methotrexate is administered to an actively dividing asynchronous culture, cells are arrested not only at the G1/S interface but also in early and middle S phase. Most interestingly, two-dimensional gel analysis of replication intermediates in the initiation locus of the amplified dihydrofolate reductase domain suggests that mimosine may actually inhibit initiation. Thus, this drug represents a new class of inhibitors that may open a window on regulatory events occurring at individual origins of replication.

Full Text

Duke Authors

Cited Authors

  • Mosca, PJ; Dijkwel, PA; Hamlin, JL

Published Date

  • October 1992

Published In

Volume / Issue

  • 12 / 10

Start / End Page

  • 4375 - 4383

PubMed ID

  • 1406627

Pubmed Central ID

  • 1406627

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.12.10.4375

Language

  • eng

Conference Location

  • United States