Perivascular tissue factor is down-regulated following cutaneous wounding: implications for bleeding in hemophilia.

Journal Article

Healing of skin wounds is delayed in hemophilia B (HB) mice. HB mice do not bleed excessively at wounding, yet rebleed hours to days later. Tissue factor (TF) expression is up-regulated by inflammatory cytokines and has been linked to angiogenesis. We hypothesized that impaired thrombin generation in HB leads to impaired TF expression following injury. Punch biopsies were placed on wild-type (WT) and HB mice. Tissues from wound sites were immunostained for TF. Blood vessels are normally surrounded by a coat of pericytes expressing TF. Surprisingly, within a day after wounding TF disappeared from around nearby vessels; returning after 8 days in WT and 10 days in HB mice. The granulation tissue filling the wound during healing also lacked TF around angiogenic vessels. Thus, perivascular TF expression is down-regulated during wound healing. This may prevent thrombosis of neovessels during angiogenesis but renders hemophiliacs vulnerable to hemorrhage during healing.

Full Text

Duke Authors

Cited Authors

  • McDonald, AG; Yang, K; Roberts, HR; Monroe, DM; Hoffman, M

Published Date

  • February 15, 2008

Published In

Volume / Issue

  • 111 / 4

Start / End Page

  • 2046 - 2048

PubMed ID

  • 18055866

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2007-05-092916

Language

  • eng

Conference Location

  • United States