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Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP.

Publication ,  Journal Article
Cheng, DY; DeWitt, BJ; McMahon, TJ; Kadowitz, PJ
Published in: Am J Physiol
June 1994

The comparative effects of the nitric oxide (NO) synthase inhibitors N omega-nitro-L-arginine (L-NNA), N omega-nitro-L-arginine methyl ester (L-NAME), and N omega-nitro-L-arginine benzyl ester (L-NABE) on baseline tone and on vasodilator responses to acetylcholine (ACh), bradykinin (BK), and substance P (SP) were compared in the pulmonary vascular bed of the cat under constant flow conditions. After administration of the NO synthase inhibitors in intravenous doses of 100 mg/kg, the increase in lobar arterial pressure and the attenuation of vasodilator responses to ACh, BK, and SP were similar, whereas responses to adenosine and felodipine, endothelium-independent vasodilator agents, were not altered. In addition to inhibiting responses to ACh, BK, and substance P, the NO synthase inhibitors enhanced vasodilator responses to S-nitroso-N-acetylpenicillamine and NO. Moreover, atropine inhibited pulmonary vasodilator responses to ACh but not to SP or BK, and L-NAME or L-NABE had no effect on the decrease in heart rate in response to efferent vagal stimulation, a muscarinic receptor-mediated response that is independent of NO release. The similar inhibitory effects of L-NNA, L-NAME, and L-NABE on vasodilator responses to ACh, BK, and SP suggest that the L-arginine analogue, L-NNA, as well as the methyl and benzyl esters of L-NNA are useful probes for studying NO-mediated endothelium-dependent responses in the pulmonary vascular bed of the intact-chest cat.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

June 1994

Volume

266

Issue

6 Pt 2

Start / End Page

H2416 / H2422

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Vasodilation
  • Substance P
  • Pulmonary Circulation
  • Nitroarginine
  • Nitric Oxide Synthase
  • NG-Nitroarginine Methyl Ester
  • Male
  • Heart Rate
  • Female
 

Citation

APA
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MLA
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Cheng, D. Y., DeWitt, B. J., McMahon, T. J., & Kadowitz, P. J. (1994). Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP. Am J Physiol, 266(6 Pt 2), H2416–H2422. https://doi.org/10.1152/ajpheart.1994.266.6.H2416
Cheng, D. Y., B. J. DeWitt, T. J. McMahon, and P. J. Kadowitz. “Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP.Am J Physiol 266, no. 6 Pt 2 (June 1994): H2416–22. https://doi.org/10.1152/ajpheart.1994.266.6.H2416.
Cheng DY, DeWitt BJ, McMahon TJ, Kadowitz PJ. Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP. Am J Physiol. 1994 Jun;266(6 Pt 2):H2416–22.
Cheng, D. Y., et al. “Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP.Am J Physiol, vol. 266, no. 6 Pt 2, June 1994, pp. H2416–22. Pubmed, doi:10.1152/ajpheart.1994.266.6.H2416.
Cheng DY, DeWitt BJ, McMahon TJ, Kadowitz PJ. Comparative effects of L-NNA and alkyl esters of L-NNA on pulmonary vasodilator responses to ACh, BK, and SP. Am J Physiol. 1994 Jun;266(6 Pt 2):H2416–H2422.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

June 1994

Volume

266

Issue

6 Pt 2

Start / End Page

H2416 / H2422

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Vasodilation
  • Substance P
  • Pulmonary Circulation
  • Nitroarginine
  • Nitric Oxide Synthase
  • NG-Nitroarginine Methyl Ester
  • Male
  • Heart Rate
  • Female