Conservation of the chitin utilization pathway in the Vibrionaceae.

Published

Journal Article

Vibrionaceae are regarded as important marine chitin degraders, and attachment to chitin regulates important biological functions; yet, the degree of chitin pathway conservation in Vibrionaceae is unknown. Here, a core chitin degradation pathway is proposed based on comparison of 19 Vibrio and Photobacterium genomes with a detailed metabolic map assembled for V. cholerae from published biochemical, genomic, and transcriptomic results. Further, to assess whether chitin degradation is a conserved property of Vibrionaceae, a set of 54 strains from 32 taxa were tested for the ability to grow on various forms of chitin. All strains grew on N-acetylglucosamine (GlcNAc), the monomer of chitin. The majority of isolates grew on alpha (crab shell) and beta (squid pen) chitin and contained chitinase A (chiA) genes. chiA sequencing and phylogenetic analysis suggest that this gene is a good indicator of chitin metabolism but appears subject to horizontal gene transfer and duplication. Overall, chitin metabolism appears to be a core function of Vibrionaceae, but individual pathway components exhibit dynamic evolutionary histories.

Full Text

Duke Authors

Cited Authors

  • Hunt, DE; Gevers, D; Vahora, NM; Polz, MF

Published Date

  • January 2008

Published In

Volume / Issue

  • 74 / 1

Start / End Page

  • 44 - 51

PubMed ID

  • 17933912

Pubmed Central ID

  • 17933912

Electronic International Standard Serial Number (EISSN)

  • 1098-5336

International Standard Serial Number (ISSN)

  • 0099-2240

Digital Object Identifier (DOI)

  • 10.1128/AEM.01412-07

Language

  • eng