Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.
Published
Journal Article
The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
Full Text
Duke Authors
Cited Authors
- Hunt, DE; Klepac-Ceraj, V; Acinas, SG; Gautier, C; Bertilsson, S; Polz, MF
Published Date
- March 2006
Published In
Volume / Issue
- 72 / 3
Start / End Page
- 2221 - 2225
PubMed ID
- 16517676
Pubmed Central ID
- 16517676
Electronic International Standard Serial Number (EISSN)
- 1098-5336
International Standard Serial Number (ISSN)
- 0099-2240
Digital Object Identifier (DOI)
- 10.1128/aem.72.3.2221-2225.2006
Language
- eng