Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.
Publication
, Journal Article
Hunt, DE; Klepac-Ceraj, V; Acinas, SG; Gautier, C; Bertilsson, S; Polz, MF
Published in: Applied and environmental microbiology
March 2006
The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Applied and environmental microbiology
DOI
EISSN
1098-5336
ISSN
0099-2240
Publication Date
March 2006
Volume
72
Issue
3
Start / End Page
2221 / 2225
Related Subject Headings
- Sequence Analysis, DNA
- RNA, Ribosomal, 23S
- Polymerase Chain Reaction
- Phylogeny
- Molecular Sequence Data
- Microbiology
- Gene Library
- DNA Primers
- Base Sequence
- Bacteria
Citation
APA
Chicago
ICMJE
MLA
NLM
Hunt, D. E., Klepac-Ceraj, V., Acinas, S. G., Gautier, C., Bertilsson, S., & Polz, M. F. (2006). Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and Environmental Microbiology, 72(3), 2221–2225. https://doi.org/10.1128/aem.72.3.2221-2225.2006
Hunt, Dana E., Vanja Klepac-Ceraj, Silvia G. Acinas, Clement Gautier, Stefan Bertilsson, and Martin F. Polz. “Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.” Applied and Environmental Microbiology 72, no. 3 (March 2006): 2221–25. https://doi.org/10.1128/aem.72.3.2221-2225.2006.
Hunt DE, Klepac-Ceraj V, Acinas SG, Gautier C, Bertilsson S, Polz MF. Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and environmental microbiology. 2006 Mar;72(3):2221–5.
Hunt, Dana E., et al. “Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.” Applied and Environmental Microbiology, vol. 72, no. 3, Mar. 2006, pp. 2221–25. Epmc, doi:10.1128/aem.72.3.2221-2225.2006.
Hunt DE, Klepac-Ceraj V, Acinas SG, Gautier C, Bertilsson S, Polz MF. Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and environmental microbiology. 2006 Mar;72(3):2221–2225.
Published In
Applied and environmental microbiology
DOI
EISSN
1098-5336
ISSN
0099-2240
Publication Date
March 2006
Volume
72
Issue
3
Start / End Page
2221 / 2225
Related Subject Headings
- Sequence Analysis, DNA
- RNA, Ribosomal, 23S
- Polymerase Chain Reaction
- Phylogeny
- Molecular Sequence Data
- Microbiology
- Gene Library
- DNA Primers
- Base Sequence
- Bacteria