Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.

Journal Article (Journal Article)

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

Full Text

Duke Authors

Cited Authors

  • Hunt, DE; Klepac-Ceraj, V; Acinas, SG; Gautier, C; Bertilsson, S; Polz, MF

Published Date

  • March 2006

Published In

Volume / Issue

  • 72 / 3

Start / End Page

  • 2221 - 2225

PubMed ID

  • 16517676

Pubmed Central ID

  • PMC1393206

Electronic International Standard Serial Number (EISSN)

  • 1098-5336

International Standard Serial Number (ISSN)

  • 0099-2240

Digital Object Identifier (DOI)

  • 10.1128/aem.72.3.2221-2225.2006

Language

  • eng