Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots.

Published

Journal Article

The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV-vis spectroscopy on whole and lysed bacterial cells. The organic-acid-stabilized QDs span a range in size from 3.8+/-1.1 to 6.0+/-2.4 nm with emission wavelengths from 540 to 630 nm. QDs of different sizes (i.e., 3.8-6 nm) can enter the bacterium and be detected on different fluorescence channels with little interference from other QDs as a result of the distinct emission profiles (i.e., 540-630 nm, respectively). Costaining QD-labeled E. coli with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) demonstrates that the QDs and DAPI are colocalized within E. coli, whereas costaining QD-labeled E. coli with membrane dye FM4-64 shows that the FM4-64 is localized in the outer bacterial membrane and that the QDs are inside.

Full Text

Duke Authors

Cited Authors

  • Hirschey, MD; Han, Y-J; Stucky, GD; Butler, A

Published Date

  • July 2006

Published In

Volume / Issue

  • 11 / 5

Start / End Page

  • 663 - 669

PubMed ID

  • 16724226

Pubmed Central ID

  • 16724226

International Standard Serial Number (ISSN)

  • 0949-8257

Digital Object Identifier (DOI)

  • 10.1007/s00775-006-0116-7

Language

  • eng

Conference Location

  • Germany