Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots.
Journal Article (Journal Article)
The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV-vis spectroscopy on whole and lysed bacterial cells. The organic-acid-stabilized QDs span a range in size from 3.8+/-1.1 to 6.0+/-2.4 nm with emission wavelengths from 540 to 630 nm. QDs of different sizes (i.e., 3.8-6 nm) can enter the bacterium and be detected on different fluorescence channels with little interference from other QDs as a result of the distinct emission profiles (i.e., 540-630 nm, respectively). Costaining QD-labeled E. coli with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) demonstrates that the QDs and DAPI are colocalized within E. coli, whereas costaining QD-labeled E. coli with membrane dye FM4-64 shows that the FM4-64 is localized in the outer bacterial membrane and that the QDs are inside.
Full Text
Duke Authors
Cited Authors
- Hirschey, MD; Han, Y-J; Stucky, GD; Butler, A
Published Date
- July 2006
Published In
Volume / Issue
- 11 / 5
Start / End Page
- 663 - 669
PubMed ID
- 16724226
International Standard Serial Number (ISSN)
- 0949-8257
Digital Object Identifier (DOI)
- 10.1007/s00775-006-0116-7
Language
- eng
Conference Location
- Germany