DNA gyrase genes in Mycobacterium tuberculosis: a single operon driven by multiple promoters.


Journal Article

The two genes encoding DNA gyrase in Mycobacterium tuberculosis are present next to each other in the genome, with gyrB upstream of gyrA. We show that the primary transcript is dicistronic. However, in addition to the principal promoter, there are multiple weaker promoters that appear to fine-tune transcription. With these and other mycobacterial promoters, we propose consensus promoter sequences for two distinct sigma factors. In addition to this, the gyr genes in M. tuberculosis, as in other species, are subject to autoregulation, albeit with slower kinetics, probably reflecting the slower metabolism of the organism.

Full Text

Cited Authors

  • Unniraman, S; Chatterji, M; Nagaraja, V

Published Date

  • October 2002

Published In

Volume / Issue

  • 184 / 19

Start / End Page

  • 5449 - 5456

PubMed ID

  • 12218033

Pubmed Central ID

  • 12218033

Electronic International Standard Serial Number (EISSN)

  • 1098-5530

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/JB.184.19.5449-5456.2002


  • eng