Functional cooperation between topoisomerase I and single strand DNA-binding protein.


Journal Article

Protein-protein interactions play important role in cell biochemistry by favorably or adversely influencing major molecular events. In most documented cases, the interaction is direct between the partner molecules. Influence of activity in the absence of direct physical interaction between DNA transaction proteins is another important means of modulation. We show here that single strand binding protein stimulates DNA topoisomerase I activity without direct protein-protein interactions. The stimulation is specific to topoisomerase I, as DNA gyrase activity is unaffected by SSB. We propose that such cases of functional collaboration between DNA transaction proteins play important roles in vivo.

Full Text

Cited Authors

  • Sikder, D; Unniraman, S; Bhaduri, T; Nagaraja, V

Published Date

  • March 2001

Published In

Volume / Issue

  • 306 / 4

Start / End Page

  • 669 - 679

PubMed ID

  • 11243779

Pubmed Central ID

  • 11243779

Electronic International Standard Serial Number (EISSN)

  • 1089-8638

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1006/jmbi.2000.4384


  • eng