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Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.

Publication ,  Journal Article
Mohs, AM; Mancini, MC; Singhal, S; Provenzale, JM; Leyland-Jones, B; Wang, MD; Nie, S
Published in: Anal Chem
November 1, 2010

Surgery is one of the most effective and widely used procedures in treating human cancers, but a major problem is that the surgeon often fails to remove the entire tumor, leaving behind tumor-positive margins, metastatic lymph nodes, and/or satellite tumor nodules. Here we report the use of a hand-held spectroscopic pen device (termed SpectroPen) and near-infrared contrast agents for intraoperative detection of malignant tumors, based on wavelength-resolved measurements of fluorescence and surface-enhanced Raman scattering (SERS) signals. The SpectroPen utilizes a near-infrared diode laser (emitting at 785 nm) coupled to a compact head unit for light excitation and collection. This pen-shaped device effectively removes silica Raman peaks from the fiber optics and attenuates the reflected excitation light, allowing sensitive analysis of both fluorescence and Raman signals. Its overall performance has been evaluated by using a fluorescent contrast agent (indocyanine green, or ICG) as well as a surface-enhanced Raman scattering (SERS) contrast agent (pegylated colloidal gold). Under in vitro conditions, the detection limits are approximately 2-5 × 10(-11) M for the indocyanine dye and 0.5-1 × 10(-13) M for the SERS contrast agent. Ex vivo tissue penetration data show attenuated but resolvable fluorescence and Raman signals when the contrast agents are buried 5-10 mm deep in fresh animal tissues. In vivo studies using mice bearing bioluminescent 4T1 breast tumors further demonstrate that the tumor borders can be precisely detected preoperatively and intraoperatively, and that the contrast signals are strongly correlated with tumor bioluminescence. After surgery, the SpectroPen device permits further evaluation of both positive and negative tumor margins around the surgical cavity, raising new possibilities for real-time tumor detection and image-guided surgery.

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Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

November 1, 2010

Volume

82

Issue

21

Start / End Page

9058 / 9065

Location

United States

Related Subject Headings

  • Swine
  • Spectrum Analysis, Raman
  • Spectrometry, Fluorescence
  • Optical Imaging
  • Mice
  • Indocyanine Green
  • Humans
  • Fluorescent Dyes
  • Female
  • Equipment Design
 

Citation

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Mohs, A. M., Mancini, M. C., Singhal, S., Provenzale, J. M., Leyland-Jones, B., Wang, M. D., & Nie, S. (2010). Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration. Anal Chem, 82(21), 9058–9065. https://doi.org/10.1021/ac102058k
Mohs, Aaron M., Michael C. Mancini, Sunil Singhal, James M. Provenzale, Brian Leyland-Jones, May D. Wang, and Shuming Nie. “Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.Anal Chem 82, no. 21 (November 1, 2010): 9058–65. https://doi.org/10.1021/ac102058k.
Mohs AM, Mancini MC, Singhal S, Provenzale JM, Leyland-Jones B, Wang MD, et al. Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration. Anal Chem. 2010 Nov 1;82(21):9058–65.
Mohs, Aaron M., et al. “Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.Anal Chem, vol. 82, no. 21, Nov. 2010, pp. 9058–65. Pubmed, doi:10.1021/ac102058k.
Mohs AM, Mancini MC, Singhal S, Provenzale JM, Leyland-Jones B, Wang MD, Nie S. Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration. Anal Chem. 2010 Nov 1;82(21):9058–9065.
Journal cover image

Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

November 1, 2010

Volume

82

Issue

21

Start / End Page

9058 / 9065

Location

United States

Related Subject Headings

  • Swine
  • Spectrum Analysis, Raman
  • Spectrometry, Fluorescence
  • Optical Imaging
  • Mice
  • Indocyanine Green
  • Humans
  • Fluorescent Dyes
  • Female
  • Equipment Design