Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.
Journal Article (Journal Article)
We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems.
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Duke Authors
Cited Authors
- Osawa, M; Erickson, HP
Published Date
- 2009
Published In
Volume / Issue
- 464 /
Start / End Page
- 3 - 17
PubMed ID
- 19903547
Pubmed Central ID
- PMC3388605
Electronic International Standard Serial Number (EISSN)
- 1557-7988
Digital Object Identifier (DOI)
- 10.1016/S0076-6879(09)64001-5
Language
- eng
Conference Location
- United States