Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.

Journal Article

We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems.

Full Text

Duke Authors

Cited Authors

  • Osawa, M; Erickson, HP

Published Date

  • 2009

Published In

Volume / Issue

  • 464 /

Start / End Page

  • 3 - 17

PubMed ID

  • 19903547

Electronic International Standard Serial Number (EISSN)

  • 1557-7988

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(09)64001-5

Language

  • eng

Conference Location

  • United States