Skip to main content
Journal cover image

Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling.

Publication ,  Journal Article
Lad, SP; Yang, G; Scott, DA; Chao, T-H; Correia, JDS; de la Torre, JC; Li, E
Published in: Mol Immunol
April 2008

The mitochondrial anti-viral signaling protein (MAVS), also known as CARDIF, IPS-1, KIAA1271 and VISA, is a mitochondria associated protein that regulates type I interferon production through coordinated activation of NF-kappaB and IRF3. The N-terminal CARD domain of MAVS interacts with RIGI helicase of upcapped RNA detection and the putative TRAF2 and TRAF6 binding motifs modulate protein interaction for NF-kappaB activation. MAVS is encoded by a single gene composed of 6 exons but is generally detected as multiple protein bands after separation by SDS-PAGE. In an effort to identify MAVS variants with diverse biological functions, we isolated three splicing variants and named them MAVS 1a (exon 2 deletion), 1b (exon 3 deletion) and 1c (exon 6 deletion), respectively. MAVS 1a and 1b, due to a frame shift by exon deletion, encode 131 and 124 aa residues, respectively. Except the first 39 aa residues encoded by exon 1, MAVS 1a does not share sequence homology with known proteins, it instead contains a putative TRAF2-binding motif and interacts with TRAF2 and RIP1. MAVS 1b shares the first 97 residues with wt MAVS and 27 aa residues of unknown protein. Unlike MAVS that activates both NF-kappaB and IRF3 pathways, expression of MAVS 1b selectively activates an IFNbeta but not an IL8 promoter. MAVS 1b interacts with RIP1 and FADD and exhibits anti-viral activity against VSV infection. This study uncovers MAVS splicing variants of diverse biological function.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Mol Immunol

DOI

ISSN

0161-5890

Publication Date

April 2008

Volume

45

Issue

8

Start / End Page

2277 / 2287

Location

England

Related Subject Headings

  • Vesiculovirus
  • Signal Transduction
  • RNA Splicing
  • Protein Structure, Tertiary
  • Protein Isoforms
  • Protein Binding
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Models, Immunological
  • Interferon-beta
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lad, S. P., Yang, G., Scott, D. A., Chao, T.-H., Correia, J. D. S., de la Torre, J. C., & Li, E. (2008). Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling. Mol Immunol, 45(8), 2277–2287. https://doi.org/10.1016/j.molimm.2007.11.018
Lad, Sonya P., Guang Yang, David A. Scott, Ta-Hsiang Chao, Jean da Silva Correia, Juan Carlos de la Torre, and Erguang Li. “Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling.Mol Immunol 45, no. 8 (April 2008): 2277–87. https://doi.org/10.1016/j.molimm.2007.11.018.
Lad SP, Yang G, Scott DA, Chao T-H, Correia JDS, de la Torre JC, et al. Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling. Mol Immunol. 2008 Apr;45(8):2277–87.
Lad, Sonya P., et al. “Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling.Mol Immunol, vol. 45, no. 8, Apr. 2008, pp. 2277–87. Pubmed, doi:10.1016/j.molimm.2007.11.018.
Lad SP, Yang G, Scott DA, Chao T-H, Correia JDS, de la Torre JC, Li E. Identification of MAVS splicing variants that interfere with RIGI/MAVS pathway signaling. Mol Immunol. 2008 Apr;45(8):2277–2287.
Journal cover image

Published In

Mol Immunol

DOI

ISSN

0161-5890

Publication Date

April 2008

Volume

45

Issue

8

Start / End Page

2277 / 2287

Location

England

Related Subject Headings

  • Vesiculovirus
  • Signal Transduction
  • RNA Splicing
  • Protein Structure, Tertiary
  • Protein Isoforms
  • Protein Binding
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Models, Immunological
  • Interferon-beta