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The presence of receptors for bombesin/GRP and mRNA for three receptor subtypes in human ovarian epithelial cancers.

Publication ,  Journal Article
Sun, B; Schally, AV; Halmos, G
Published in: Regul Pept
June 30, 2000

Bombesin-like peptides can function as autocrine or paracrine growth factors and stimulate the growth of various cancers. The antagonists of bombesin/gastrin-releasing peptide (GRP) suppress the proliferation of diverse tumors including ovarian cancer by mechanisms likely mediated by bombesin receptors. In this study, we used the reverse transcription-polymerase chain reaction (RT-PCR) method to evaluate the mRNA expression of three bombesin receptor subtypes: gastrin-releasing peptide receptor (GRPR), neuromedin B receptor (NMBR), and bombesin receptor subtype 3 (BRS-3), in 22 specimens of human epithelial ovarian cancer and in two human ovarian cancer lines. Of the 22 ovarian cancer specimens analyzed, 17 tumors ( approximately 77%) expressed mRNA for GRPR, 19 ( approximately 86%) showed NMBR mRNA and six ( approximately 27%) revealed BRS-3 mRNA. Thus, 14 of 22 specimens ( approximately 64%) expressed mRNAs for both GRPR and NMBR, and five ( approximately 23%) expressed all three subtypes. The expression of GRPR appeared to be greater in poorly differentiated ovarian carcinomas. A higher incidence of BRS-3 expression was observed in samples with tumor Stage IV (4/4, 100%) compared with Stage III (1/17, approximately 6%). mRNA for both GRPR and NMBR was also detected in OV-1063 and UCI-107 human ovarian cancer xenografts, but BRS-3 was found only in OV-1063, which originated from a metastatic tumor. In addition, functional receptors for bombesin/GRP were found in eight of 11 ovarian cancer specimens investigated and in both ovarian cancer lines by receptor binding assay. Our study indicates that GRPR and NMBR are widely distributed in human ovarian carcinomas with BRS-3 being found in Stage IV tumors. Some approaches based on bombesin/GRP receptor antagonists or targeted bombesin analogs could be considered for treatment of ovarian cancers.

Duke Scholars

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Published In

Regul Pept

DOI

ISSN

0167-0115

Publication Date

June 30, 2000

Volume

90

Issue

1-3

Start / End Page

77 / 84

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Radioligand Assay
  • RNA, Messenger
  • Ovary
  • Ovarian Neoplasms
  • Neoplasms, Glandular and Epithelial
  • Middle Aged
  • Mice, Nude
  • Mice
 

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Sun, B., Schally, A. V., & Halmos, G. (2000). The presence of receptors for bombesin/GRP and mRNA for three receptor subtypes in human ovarian epithelial cancers. Regul Pept, 90(1–3), 77–84. https://doi.org/10.1016/s0167-0115(00)00114-2
Sun, B., A. V. Schally, and G. Halmos. “The presence of receptors for bombesin/GRP and mRNA for three receptor subtypes in human ovarian epithelial cancers.Regul Pept 90, no. 1–3 (June 30, 2000): 77–84. https://doi.org/10.1016/s0167-0115(00)00114-2.
Sun, B., et al. “The presence of receptors for bombesin/GRP and mRNA for three receptor subtypes in human ovarian epithelial cancers.Regul Pept, vol. 90, no. 1–3, June 2000, pp. 77–84. Pubmed, doi:10.1016/s0167-0115(00)00114-2.
Journal cover image

Published In

Regul Pept

DOI

ISSN

0167-0115

Publication Date

June 30, 2000

Volume

90

Issue

1-3

Start / End Page

77 / 84

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Radioligand Assay
  • RNA, Messenger
  • Ovary
  • Ovarian Neoplasms
  • Neoplasms, Glandular and Epithelial
  • Middle Aged
  • Mice, Nude
  • Mice