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Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238.

Publication ,  Journal Article
Kiaris, H; Schally, AV; Nagy, A; Sun, B; Szepeshazi, K; Halmos, G
Published in: Clin Cancer Res
February 2000

Receptors for somatostatin (SST) found on brain tumors could be used for targeting of chemotherapeutic agents. This study was conducted to investigate the effects of targeted cytotoxic SST analogue AN-238, consisting of 2-pyrrolinodoxorubicin (AN-201), a potent derivative of doxorubicin (DOX) linked to somatostatin analogue RC-121, on the growth of SST receptor-positive U-87 MG human glioblastomas. Nude mice bearing U-87 MG xenografts received i.v. saline or equimolar doses of AN-238 or AN-201 (150 nmol/kg). Experiments also included groups that were administered RC-121 prior to the injection of AN-238, and groups injected with AN-162, a cytotoxic SST analogue similar to AN-238 but containing DOX instead of AN-201. Tumor volume, weight, and burden were determined. The effect of AN-238 and AN-201 on the survival time of nude mice bearing orthotopically implanted U-87 MG tumors was also evaluated. The binding of AN-238 to U-87 MG tumors was determined by radioreceptor assay and SST receptor (SSTR) subtype by reverse transcription-PCR. Nineteen days after a single administration of AN-238 the growth of U-87 MG tumors in nude mice was significantly inhibited (P = 0.00168), whereas two injections of AN-238 produced the regression of tumors (P = 0.0046). AN-201 was toxic and ineffective at the same dose. The antitumor effect on AN-238 could be blocked by pretreatment of the tumor-bearing mice with RC-121. The mean survival time of nude mice inoculated orthotopically with U-87 MG cells into the brain was significantly prolonged by treatment with AN-238 (P = 0.0099). AN-162 failed to inhibit significantly the growth of U-87 MG xenografts. High affinity binding sites for SST and mRNA for SST-2 receptor subtype were detected in U-87 MG tumors. Cytotoxic SST analogue AN-238 can be targeted to SST receptors on U-87 MG human glioblastomas to produce powerful inhibition of growth.

Duke Scholars

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

February 2000

Volume

6

Issue

2

Start / End Page

709 / 717

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Pyrroles
  • Oncology & Carcinogenesis
  • Octreotide
  • Mice, Nude
  • Mice
  • Male
  • Humans
  • Glioblastoma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kiaris, H., Schally, A. V., Nagy, A., Sun, B., Szepeshazi, K., & Halmos, G. (2000). Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238. Clin Cancer Res, 6(2), 709–717.
Kiaris, H., A. V. Schally, A. Nagy, B. Sun, K. Szepeshazi, and G. Halmos. “Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238.Clin Cancer Res 6, no. 2 (February 2000): 709–17.
Kiaris H, Schally AV, Nagy A, Sun B, Szepeshazi K, Halmos G. Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238. Clin Cancer Res. 2000 Feb;6(2):709–17.
Kiaris, H., et al. “Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238.Clin Cancer Res, vol. 6, no. 2, Feb. 2000, pp. 709–17.
Kiaris H, Schally AV, Nagy A, Sun B, Szepeshazi K, Halmos G. Regression of U-87 MG human glioblastomas in nude mice after treatment with a cytotoxic somatostatin analog AN-238. Clin Cancer Res. 2000 Feb;6(2):709–717.

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

February 2000

Volume

6

Issue

2

Start / End Page

709 / 717

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Pyrroles
  • Oncology & Carcinogenesis
  • Octreotide
  • Mice, Nude
  • Mice
  • Male
  • Humans
  • Glioblastoma