Skip to main content
Journal cover image

Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer.

Publication ,  Journal Article
Darling, EM; Pritchett, PE; Evans, BA; Superfine, R; Zauscher, S; Guilak, F
Published in: Cellular and molecular bioengineering
August 2009

Chondrocytes in articular cartilage normally exhibit high expression of collagen II and aggrecan but rapidly dedifferentiate to a fibroblastic phenotype if passaged in culture. Previous studies have suggested that the loss of chondrocyte phenotype is associated with changes in the structure of the F-actin cytoskeleton, which also controls cell mechanical properties. In this study, we examined how dedifferentiation in monolayer influences the mechanical properties of chondrocytes isolated from different zones of articular cartilage. Atomic force microscopy was used to measure the mechanical properties of superficial and middle/deep zone chondrocytes as they underwent serial passaging and subsequent growth on fibronectin-coated, micropatterned self-assembled monolayers (MSAMs) that restored a rounded cell shape in 2D culture. Chondrocytes exhibited significant increases in elastic and viscoelastic moduli with dedifferentiation in culture. These changes were only partially ameliorated by the restoration of a rounded shape on micropatterned surfaces. Furthermore, intrinsic zonal differences in cell mechanical properties were rapidly lost with passage. These findings indicate that cell mechanical properties may provide additional measures of phenotypic expression of chondrocytes as they undergo dedifferentiation and possibly redifferentiation in culture.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cellular and molecular bioengineering

DOI

EISSN

1865-5033

ISSN

1865-5025

Publication Date

August 2009

Volume

2

Issue

3

Start / End Page

395 / 404

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Darling, E. M., Pritchett, P. E., Evans, B. A., Superfine, R., Zauscher, S., & Guilak, F. (2009). Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer. Cellular and Molecular Bioengineering, 2(3), 395–404. https://doi.org/10.1007/s12195-009-0077-3
Darling, Eric M., Poston E. Pritchett, Benjamin A. Evans, Richard Superfine, Stefan Zauscher, and Farshid Guilak. “Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer.Cellular and Molecular Bioengineering 2, no. 3 (August 2009): 395–404. https://doi.org/10.1007/s12195-009-0077-3.
Darling EM, Pritchett PE, Evans BA, Superfine R, Zauscher S, Guilak F. Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer. Cellular and molecular bioengineering. 2009 Aug;2(3):395–404.
Darling, Eric M., et al. “Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer.Cellular and Molecular Bioengineering, vol. 2, no. 3, Aug. 2009, pp. 395–404. Epmc, doi:10.1007/s12195-009-0077-3.
Darling EM, Pritchett PE, Evans BA, Superfine R, Zauscher S, Guilak F. Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer. Cellular and molecular bioengineering. 2009 Aug;2(3):395–404.
Journal cover image

Published In

Cellular and molecular bioengineering

DOI

EISSN

1865-5033

ISSN

1865-5025

Publication Date

August 2009

Volume

2

Issue

3

Start / End Page

395 / 404

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering