Skip to main content

The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage.

Publication ,  Journal Article
Shen, S; Zhu, M; Lau, J; Chuck, M; Zhang, W
Published in: J Immunol
May 1, 2009

The linker for activation of T cells (LAT) is an adaptor protein that couples TCR engagement to downstream signaling cascades. LAT is important in early thymocyte development as LAT-deficient mice have a complete block at the double-negative (DN) 3 stage. To study the role of LAT beyond the DN3 stage, we generated mice in which the lat gene could be deleted by the Cre recombinase. Analysis of these mice showed that deletion of LAT after the DN3 stage allowed thymocytes to develop past the DN3 to DN4 checkpoint and to generate double-positive thymocytes. However, LAT-deficient DP thymocytes were severely defective in responding to stimulation via the TCR and failed to differentiate into single-positive thymocytes efficiently. Consequently, few LAT-deficient mature T cells could be found in the periphery. These T cells had undergone extensive homeostatic proliferation and expressed low levels of the TCR on their surface. Collectively, our data indicate that in addition to its role in pre-TCR signaling, LAT also plays an essential role in thymocyte development during transition from the double-positive to single-positive stage.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

May 1, 2009

Volume

182

Issue

9

Start / End Page

5596 / 5604

Location

United States

Related Subject Headings

  • Thymus Gland
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Phosphoproteins
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shen, S., Zhu, M., Lau, J., Chuck, M., & Zhang, W. (2009). The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage. J Immunol, 182(9), 5596–5604. https://doi.org/10.4049/jimmunol.0803170
Shen, Shudan, Minghua Zhu, Jasmine Lau, Mariana Chuck, and Weiguo Zhang. “The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage.J Immunol 182, no. 9 (May 1, 2009): 5596–5604. https://doi.org/10.4049/jimmunol.0803170.
Shen S, Zhu M, Lau J, Chuck M, Zhang W. The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage. J Immunol. 2009 May 1;182(9):5596–604.
Shen, Shudan, et al. “The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage.J Immunol, vol. 182, no. 9, May 2009, pp. 5596–604. Pubmed, doi:10.4049/jimmunol.0803170.
Shen S, Zhu M, Lau J, Chuck M, Zhang W. The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage. J Immunol. 2009 May 1;182(9):5596–5604.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

May 1, 2009

Volume

182

Issue

9

Start / End Page

5596 / 5604

Location

United States

Related Subject Headings

  • Thymus Gland
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Phosphoproteins
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins