Split tolerance in peripheral B cell subsets in mice expressing a low level of Igkappa-reactive ligand.

Published

Journal Article

Peripheral B cell tolerance differs from central tolerance in anatomic location, in the stage of B cell development, and in the diversity of Ag-responsive cells. B cells in secondary lymphoid organs are heterogeneous, including numerous subtypes such as B-1, marginal zone, transitional, and follicular B cells, which likely respond differently from one another to ligand encounter. We showed recently that central B cell tolerance mediated by receptor editing was induced in mice carrying high levels of a ubiquitously expressed kappa-macroself Ag, a synthetic superantigen reactive to Igkappa. In this study, we characterize a new transgenic line that has a distinctly lower expression pattern from those described previously; the B cell tolerance phenotype of these mice is characterized by the presence of significant numbers of immature kappa+ B cells in the spleen, the loss of mature follicular and marginal zone B cells, the persistence of kappa+ B-1 cells in the peritoneal cavity, and significant levels of serum IgM,kappa. These findings suggest distinct signaling thresholds for tolerance among peripheral B cell subsets reactive with an identical ligand.

Full Text

Duke Authors

Cited Authors

  • Aït-Azzouzene, D; Verkoczy, L; Duong, B; Skog, P; Gavin, AL; Nemazee, D

Published Date

  • January 15, 2006

Published In

Volume / Issue

  • 176 / 2

Start / End Page

  • 939 - 948

PubMed ID

  • 16393979

Pubmed Central ID

  • 16393979

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.176.2.939

Language

  • eng

Conference Location

  • United States