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Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1.

Publication ,  Journal Article
McNulty, AL; Estes, BT; Wilusz, RE; Weinberg, JB; Guilak, F
Published in: Osteoarthritis Cartilage
June 2010

OBJECTIVE: Meniscal tears are a common knee injury and increased levels of interleukin-1 (IL-1) have been measured in injured and degenerated joints. Studies have shown that IL-1 decreases the shear strength, cell accumulation, and tissue formation in meniscal repair interfaces. While mechanical stress and IL-1 modulate meniscal biosynthesis and degradation, the effects of dynamic loading on meniscal repair are unknown. The purpose of this study was to determine the effects of mechanical compression on meniscal repair under normal and inflammatory conditions. EXPERIMENTAL DESIGN: Explants were harvested from porcine medial menisci. To simulate a full-thickness defect, a central core was removed and reinserted. Explants were loaded for 4h/day at 1 Hz and 0%-26% strain for 14 days in the presence of 0 or 100 pg/mL of IL-1. Media were assessed for matrix metalloproteinase (MMP) activity, aggrecanase activity, sulfated glycosaminoglycan (S-GAG) release, and nitric oxide (NO) production. After 14 days, biomechanical testing and histological analyses were performed. RESULTS: IL-1 increased MMP activity, S-GAG release, and NO production, while decreasing the shear strength and tissue repair in the interface. Dynamic loading antagonized IL-1-mediated inhibition of repair at all strain amplitudes. Neither IL-1 treatment nor strain altered aggrecanase activity. Additionally, strain alone did not alter meniscal healing, except at the highest strain magnitude (26%), a level that enhanced the strength of repair. CONCLUSIONS: Dynamic loading blocked the catabolic effects of IL-1 on meniscal repair, suggesting that joint loading through physical therapy may be beneficial in promoting healing of meniscal lesions under inflammatory conditions.

Duke Scholars

Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

June 2010

Volume

18

Issue

6

Start / End Page

830 / 838

Location

England

Related Subject Headings

  • Wound Healing
  • Tibial Meniscus Injuries
  • Swine
  • Stress, Mechanical
  • Nitric Oxide
  • Menisci, Tibial
  • Matrix Metalloproteinases
  • Knee Injuries
  • Interleukin-1
  • Glycosaminoglycans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McNulty, A. L., Estes, B. T., Wilusz, R. E., Weinberg, J. B., & Guilak, F. (2010). Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1. Osteoarthritis Cartilage, 18(6), 830–838. https://doi.org/10.1016/j.joca.2010.02.009
McNulty, A. L., B. T. Estes, R. E. Wilusz, J. B. Weinberg, and F. Guilak. “Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1.Osteoarthritis Cartilage 18, no. 6 (June 2010): 830–38. https://doi.org/10.1016/j.joca.2010.02.009.
McNulty AL, Estes BT, Wilusz RE, Weinberg JB, Guilak F. Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1. Osteoarthritis Cartilage. 2010 Jun;18(6):830–8.
McNulty, A. L., et al. “Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1.Osteoarthritis Cartilage, vol. 18, no. 6, June 2010, pp. 830–38. Pubmed, doi:10.1016/j.joca.2010.02.009.
McNulty AL, Estes BT, Wilusz RE, Weinberg JB, Guilak F. Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1. Osteoarthritis Cartilage. 2010 Jun;18(6):830–838.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

June 2010

Volume

18

Issue

6

Start / End Page

830 / 838

Location

England

Related Subject Headings

  • Wound Healing
  • Tibial Meniscus Injuries
  • Swine
  • Stress, Mechanical
  • Nitric Oxide
  • Menisci, Tibial
  • Matrix Metalloproteinases
  • Knee Injuries
  • Interleukin-1
  • Glycosaminoglycans