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Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs.

Publication ,  Journal Article
Mahmoud, TH; McCuen, BW; Hao, Y; Moon, SJ; Tatebayashi, M; Stinnett, S; Petters, RM; Wong, F
Published in: Graefes Arch Clin Exp Ophthalmol
April 2003

BACKGROUND: Photoreceptor degeneration in retinitis pigmentosa (RP) runs an inevitable, gradually progressive course. A wide variety of growth factors of different origins have been shown to slow the rate of degeneration in some rodent models of RP. Recently, lens-derived neurotrophic factors have been shown to rescue degenerating ganglion cells in crush models of the optic nerve. Our objective was to evaluate the potential rescue effect of lensectomy and vitrectomy (L&V) on photoreceptor degeneration in a large-animal model, the rhodopsin P347L transgenic pig. METHODS: We operated on one eye of each of 49 3-week-old pigs--15 vitrectomies and 34 L&V, 6 of which received steroids. Retinal paraffin sections were prepared for all eyes, in addition to immunohistochemistry in four eyes, 8 weeks after L&V. RESULTS: At eight weeks after L&V, operated eyes showed significantly more nuclei in the outer nuclear layer (ONL) than the unoperated fellow eyes. The better preservation of the ONL persisted but was less prominent by 20 weeks after surgery. Steroid treatment did not markedly reduce the better preservation of the ONL seen at 8, 10, and 12 weeks after surgery. The significant difference in cell count between operated and unoperated eyes in the L&V group at 8 weeks was due to the difference in the number of rods, not the cones. CONCLUSION: Lensectomy and vitrectomy delay photoreceptor degeneration in rhodopsin P347L transgenic pigs. Lens-related rescue effect is a probable reason for the delayed degeneration.

Duke Scholars

Published In

Graefes Arch Clin Exp Ophthalmol

DOI

ISSN

0721-832X

Publication Date

April 2003

Volume

241

Issue

4

Start / End Page

298 / 308

Location

Germany

Related Subject Headings

  • Vitrectomy
  • Rhodopsin
  • Retinal Degeneration
  • Photoreceptor Cells, Vertebrate
  • Ophthalmology & Optometry
  • Lens, Crystalline
  • Fluorescent Antibody Technique, Indirect
  • Disease Models, Animal
  • Cell Count
  • Animals, Genetically Modified
 

Citation

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MLA
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Mahmoud, T. H., McCuen, B. W., Hao, Y., Moon, S. J., Tatebayashi, M., Stinnett, S., … Wong, F. (2003). Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs. Graefes Arch Clin Exp Ophthalmol, 241(4), 298–308. https://doi.org/10.1007/s00417-003-0637-5
Mahmoud, Tamer H., Brooks W. McCuen, Ying Hao, Suk J. Moon, Misako Tatebayashi, Sandra Stinnett, Robert M. Petters, and Fulton Wong. “Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs.Graefes Arch Clin Exp Ophthalmol 241, no. 4 (April 2003): 298–308. https://doi.org/10.1007/s00417-003-0637-5.
Mahmoud TH, McCuen BW, Hao Y, Moon SJ, Tatebayashi M, Stinnett S, et al. Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs. Graefes Arch Clin Exp Ophthalmol. 2003 Apr;241(4):298–308.
Mahmoud, Tamer H., et al. “Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs.Graefes Arch Clin Exp Ophthalmol, vol. 241, no. 4, Apr. 2003, pp. 298–308. Pubmed, doi:10.1007/s00417-003-0637-5.
Mahmoud TH, McCuen BW, Hao Y, Moon SJ, Tatebayashi M, Stinnett S, Petters RM, Wong F. Lensectomy and vitrectomy decrease the rate of photoreceptor loss in rhodopsin P347L transgenic pigs. Graefes Arch Clin Exp Ophthalmol. 2003 Apr;241(4):298–308.
Journal cover image

Published In

Graefes Arch Clin Exp Ophthalmol

DOI

ISSN

0721-832X

Publication Date

April 2003

Volume

241

Issue

4

Start / End Page

298 / 308

Location

Germany

Related Subject Headings

  • Vitrectomy
  • Rhodopsin
  • Retinal Degeneration
  • Photoreceptor Cells, Vertebrate
  • Ophthalmology & Optometry
  • Lens, Crystalline
  • Fluorescent Antibody Technique, Indirect
  • Disease Models, Animal
  • Cell Count
  • Animals, Genetically Modified