Determination of Cloretazine (VNP40101M) and its active metabolite (VNP4090CE) in human plasma by liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS).


Journal Article

A sensitive method for the determination of Cloretazine (VNP40101M) and its metabolite (VNP4090CE) with an internal standard (ISTD) in human plasma was developed using high-performance liquid chromatographic separation with tandem mass spectrometric detection. Acidified plasma samples (500 microL) were prepared using solid phase extraction (SPE) columns, and 25 microL of the reconstituted sample was injected onto an Ascentis C18 HPLC column (3 microm, 5 cmx2.1 mm) with an isocratic mobile phase. Analytes were detected with an API-3000 LC-MS/MS System at unit (Q1) and low (Q3) resolution in negative multiple reaction monitoring mode: m/z 249.0 (precursor ion) to m/z 114.9 (product ion) for both Cloretazine (at 3.64 min) and VNP4090CE (at 2.91 min), and m/z 253.0 (precursor ion) to m/z 116.9 (product ion) for the ISTD. The mean recovery for Cloretazine (VNP40101M) and its metabolite (VNP4090CE) was greater than 87% with a lower limit of quantification of 1.0 ng/mL for Cloretazine (S/N=9.7, CV

Full Text

Cited Authors

  • Bai, F; Minkin, P; Fraga, CH; O'Shaughnessy, MA; Gururangan, S; Stewart, CF

Published Date

  • June 2007

Published In

Volume / Issue

  • 853 / 1-2

Start / End Page

  • 97 - 104

PubMed ID

  • 17446145

Pubmed Central ID

  • 17446145

Electronic International Standard Serial Number (EISSN)

  • 1873-376X

International Standard Serial Number (ISSN)

  • 1570-0232

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2007.02.062


  • eng