Skip to main content
Journal cover image

Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study.

Publication ,  Journal Article
Rajagopal, MU; Hathout, Y; MacDonald, TJ; Kieran, MW; Gururangan, S; Blaney, SM; Phillips, P; Packer, R; Gordish-Dressman, H; Rood, BR
Published in: Proteomics
March 2011

The aims of this study were to demonstrate the feasibility of centrally collecting and processing high-quality cerebrospinal fluid (CSF) samples for proteomic studies within a multi-center consortium and to identify putative biomarkers for medulloblastoma in CSF. We used 2-DE to investigate the CSF proteome from 33 children with medulloblastoma and compared it against the CSF proteome from 25 age-matched controls. Protein spots were subsequently identified by a combination of in-gel tryptic digestion and MALDI-TOF TOF MS analysis. On average, 160 protein spots were detected by 2-DE and 76 protein spots corresponding to 25 unique proteins were identified using MALDI-TOF. Levels of prostaglandin D2 synthase (PGD2S) were found to be six-fold decreased in the tumor samples versus control samples (p<0.00001). These data were further validated using ELISA. Close examination of PGD2S spots revealed the presence of complex sialylated carbohydrates at residues Asn(78) and Asn(87) . Total PGD2S levels are reduced six-fold in the CSF of children with medulloblastoma most likely representing a host response to the presence of the tumor. In addition, our results demonstrate the feasibility of performing proteomic studies on CSF samples collected from patients at multiple institutions within the consortium setting.

Published In

Proteomics

DOI

EISSN

1615-9861

ISSN

1615-9853

Publication Date

March 2011

Volume

11

Issue

5

Start / End Page

935 / 943

Related Subject Headings

  • Trypsin
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Proteomics
  • Proteome
  • Protein Isoforms
  • Pediatrics
  • Molecular Sequence Data
  • Medulloblastoma
  • Lipocalins
  • Intramolecular Oxidoreductases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rajagopal, M. U., Hathout, Y., MacDonald, T. J., Kieran, M. W., Gururangan, S., Blaney, S. M., … Rood, B. R. (2011). Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study. Proteomics, 11(5), 935–943. https://doi.org/10.1002/pmic.201000198
Rajagopal, Meena U., Yetrib Hathout, Tobey J. MacDonald, Mark W. Kieran, Sri Gururangan, Susan M. Blaney, Peter Phillips, Roger Packer, Heather Gordish-Dressman, and Brian R. Rood. “Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study.Proteomics 11, no. 5 (March 2011): 935–43. https://doi.org/10.1002/pmic.201000198.
Rajagopal MU, Hathout Y, MacDonald TJ, Kieran MW, Gururangan S, Blaney SM, et al. Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study. Proteomics. 2011 Mar;11(5):935–43.
Rajagopal, Meena U., et al. “Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study.Proteomics, vol. 11, no. 5, Mar. 2011, pp. 935–43. Epmc, doi:10.1002/pmic.201000198.
Rajagopal MU, Hathout Y, MacDonald TJ, Kieran MW, Gururangan S, Blaney SM, Phillips P, Packer R, Gordish-Dressman H, Rood BR. Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study. Proteomics. 2011 Mar;11(5):935–943.
Journal cover image

Published In

Proteomics

DOI

EISSN

1615-9861

ISSN

1615-9853

Publication Date

March 2011

Volume

11

Issue

5

Start / End Page

935 / 943

Related Subject Headings

  • Trypsin
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Proteomics
  • Proteome
  • Protein Isoforms
  • Pediatrics
  • Molecular Sequence Data
  • Medulloblastoma
  • Lipocalins
  • Intramolecular Oxidoreductases