Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.

Published

Journal Article

A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands of Mr 43,000 and 72-80,000. We used the 1B10 antibody with complement to eliminate most 1B10 positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us to grow highly enriched populations of human TE cells.

Full Text

Duke Authors

Cited Authors

  • Singer, KH; Scearce, RM; Tuck, DT; Whichard, LP; Denning, SM; Haynes, BF

Published Date

  • February 1989

Published In

Volume / Issue

  • 92 / 2

Start / End Page

  • 166 - 170

PubMed ID

  • 2918230

Pubmed Central ID

  • 2918230

Electronic International Standard Serial Number (EISSN)

  • 1523-1747

International Standard Serial Number (ISSN)

  • 0022-202X

Digital Object Identifier (DOI)

  • 10.1111/1523-1747.ep12276685

Language

  • eng