Combined chemoseparation and immunoseparation of clonogenic T lymphoma cells from human bone marrow using 2'-deoxycoformycin, deoxyadenosine, 3A1 monoclonal antibody, and complement.

Journal Article (Journal Article)

Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 microM 2'-deoxycoformycin and 500 microM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-forming erythroid units, or multipotential erythroid-granulocyte-megakaryocyte-macrophage colony-forming hematopoietic progenitor cells units.

Full Text

Duke Authors

Cited Authors

  • Haleem, A; Kurtzberg, J; Olsen, GA; Rhinehardt-Clark, A; Leslie, DS; Ray, L; Smith, CA; Peters, WP; Haynes, BF; Bast, RC

Published Date

  • September 1, 1987

Published In

Volume / Issue

  • 47 / 17

Start / End Page

  • 4608 - 4612

PubMed ID

  • 3113721

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States