Combined chemoseparation and immunoseparation of clonogenic T lymphoma cells from human bone marrow using 2'-deoxycoformycin, deoxyadenosine, 3A1 monoclonal antibody, and complement.
Journal Article (Journal Article)
Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 microM 2'-deoxycoformycin and 500 microM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-forming erythroid units, or multipotential erythroid-granulocyte-megakaryocyte-macrophage colony-forming hematopoietic progenitor cells units.
Full Text
Duke Authors
Cited Authors
- Haleem, A; Kurtzberg, J; Olsen, GA; Rhinehardt-Clark, A; Leslie, DS; Ray, L; Smith, CA; Peters, WP; Haynes, BF; Bast, RC
Published Date
- September 1, 1987
Published In
Volume / Issue
- 47 / 17
Start / End Page
- 4608 - 4612
PubMed ID
- 3113721
International Standard Serial Number (ISSN)
- 0008-5472
Language
- eng
Conference Location
- United States