Skip to main content

Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope gp120 T helper cell sites and B cell neutralization epitopes.

Publication ,  Journal Article
Palker, TJ; Matthews, TJ; Langlois, A; Tanner, ME; Martin, ME; Scearce, RM; Kim, JE; Berzofsky, JA; Bolognesi, DP; Haynes, BF
Published in: J Immunol
May 15, 1989

In previous studies, we have used antisera raised to envelope (env)-gene-encoded synthetic peptides to identify a region of (HIV) glycoprotein (gp) 120 env protein designated SP10 that contains a type-specific neutralizing determinant. To develop a polyvalent, synthetic peptide inoculum that can evoke both neutralizing antibodies and T cell proliferative responses to more than one HIV isolate, synthetic peptides containing type-specific neutralizing determinants of gp120 from HIV isolates HTLV-IIIB (IIIB), HTLV-IIIMN (MN) and HTLV-IIIRF (RF) were coupled to a 16 amino acid T cell epitope (T1) of HIV-IIIB gp120 and used to immunize goats. Goat antisera to each T1-SP10 peptide derived from the SP10 region of gp120 of IIIB, MN, and RF neutralized HIV isolates IIIB, MN and RF in a type-specific manner. Moreover, peripheral blood T cells from immunized goats also proliferated in a type-specific manner to peptides derived from gp120 of IIIB, MN, and RF. When combined in a trivalent inoculum, T1-SP10 peptides from HIV-1 isolates IIIB, MN, and RF evoked a high titered neutralizing antibody response to isolates IIIB, MN, and RF in goats and as well induced immune T cells to undergo blast transformation in the presence of peptides derived from gp120 of all three HIV isolates. The T1 portion of the T1-SP10 construct was shown to induce a B cell antibody response against determinants within the T1 peptide in addition to inducing T cell proliferative responses in immune goat T cells. Moreover, the SP10 portion of the T1-SP10 constructs not only induced B cell antibody production but also induced type-specific T cell proliferative responses localized to the C-terminal variable sequences of the SP10 peptides. Finally, the T1-SP10 peptide construct induced memory T cell proliferative responses to native gp120 env protein. Thus, combinations of homologous SP10 region synthetic peptides containing type-specific neutralizing determinants and T cell epitopes of HIV gp120 may be useful in man to elicit high titered neutralizing B cell responses and, as well, T cell responses to more than one HIV isolate.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

May 15, 1989

Volume

142

Issue

10

Start / End Page

3612 / 3619

Location

United States

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Retroviridae Proteins
  • Recombinant Proteins
  • Peptide Fragments
  • Neutralization Tests
  • Molecular Sequence Data
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Immunologic Memory
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Palker, T. J., Matthews, T. J., Langlois, A., Tanner, M. E., Martin, M. E., Scearce, R. M., … Haynes, B. F. (1989). Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope gp120 T helper cell sites and B cell neutralization epitopes. J Immunol, 142(10), 3612–3619.
Palker, T. J., T. J. Matthews, A. Langlois, M. E. Tanner, M. E. Martin, R. M. Scearce, J. E. Kim, J. A. Berzofsky, D. P. Bolognesi, and B. F. Haynes. “Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope gp120 T helper cell sites and B cell neutralization epitopes.J Immunol 142, no. 10 (May 15, 1989): 3612–19.
Palker TJ, Matthews TJ, Langlois A, Tanner ME, Martin ME, Scearce RM, et al. Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope gp120 T helper cell sites and B cell neutralization epitopes. J Immunol. 1989 May 15;142(10):3612–9.
Palker TJ, Matthews TJ, Langlois A, Tanner ME, Martin ME, Scearce RM, Kim JE, Berzofsky JA, Bolognesi DP, Haynes BF. Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope gp120 T helper cell sites and B cell neutralization epitopes. J Immunol. 1989 May 15;142(10):3612–3619.

Published In

J Immunol

ISSN

0022-1767

Publication Date

May 15, 1989

Volume

142

Issue

10

Start / End Page

3612 / 3619

Location

United States

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Retroviridae Proteins
  • Recombinant Proteins
  • Peptide Fragments
  • Neutralization Tests
  • Molecular Sequence Data
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Immunologic Memory