Antibody against T lymphoblastic leukemia-associated antigen (3-40) identifies vimentin and keratin intermediate filaments in normal cells.

Journal Article (Journal Article)

Antibody 3-40 defines a 35- to 40-kd surface antigen present on T lymphoblastic leukemia (T-ALL) cells that is absent on normal hematopoietic cells (Naito et al, Blood 62:852, 1983). Using immunoblot analysis of cytoskeletal proteins and indirect immunofluorescence of cell lines treated with various cytoskeletal inhibitors, in this report we have demonstrated that antibody 3-40 also identifies vimentin intermediate filaments (IMF) within the HSB-2 (T-ALL) cell line as well as in normal thymocytes, peripheral blood mononuclear cells, and human and rodent fibroblast cell lines. Cross-reactivity with several keratin subclasses was demonstrated in both human and rodent epithelial cell lines, human thymus, and skin. In addition, we have shown that antibody 3-40 defined a 39-kd intracellular IMF-associated protein in HSB-2 cells, epithelial and fibroblast cell lines. This IMF-associated protein may be selectively expressed on the surface of human T cells during malignant transformation.

Full Text

Duke Authors

Cited Authors

  • Laster, AJ; Palker, TJ; Harden, EA; Ho, W; Naito, K; DuPont, B; Haynes, BF

Published Date

  • September 1985

Published In

Volume / Issue

  • 66 / 3

Start / End Page

  • 642 - 648

PubMed ID

  • 2411311

International Standard Serial Number (ISSN)

  • 0006-4971


  • eng

Conference Location

  • United States