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Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus.

Publication ,  Journal Article
Markert, ML; Kostyu, DD; Ward, FE; McLaughlin, TM; Watson, TJ; Buckley, RH; Schiff, SE; Ungerleider, RM; Gaynor, JW; Oldham, KT; Mahaffey, SM ...
Published in: J Immunol
January 15, 1997

Transplantation of cultured postnatal human thymus was performed in a patient with complete DiGeorge syndrome. Biopsy of the graft 3 mo after implantation revealed normal CD1+ thymocytes in thymic cortical epithelial regions and CD1- thymocytes in thymic medullary epithelial regions, respectively. HLA analysis of graft thymocyte and thymic microenvironment components demonstrated that developing thymocytes and thymic macrophages were recipient derived, while thymic epithelial components were of donor origin. The patient, who initially had no T cells and had profoundly defective T cell function, developed normal T cell responses to mitogens and Ags, tolerance to donor in a mixed lymphocyte reaction, and normal Ab titers after tetanus toxoid and pneumovax immunization. Thus, transplantation of cultured postnatal human thymic tissue in humans can form functional chimeric thymic tissue, and may provide a strategy to reconstitute the peripheral T cell pool in select congenital and acquired immune deficiency syndromes.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

January 15, 1997

Volume

158

Issue

2

Start / End Page

998 / 1005

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Thymus Gland
  • Organ Culture Techniques
  • Infant
  • Immunology
  • Humans
  • Graft Survival
  • DiGeorge Syndrome
  • Chimera
  • 3204 Immunology
 

Citation

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Markert, M. L., Kostyu, D. D., Ward, F. E., McLaughlin, T. M., Watson, T. J., Buckley, R. H., … Haynes, B. F. (1997). Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus. J Immunol, 158(2), 998–1005.
Markert, M. L., D. D. Kostyu, F. E. Ward, T. M. McLaughlin, T. J. Watson, R. H. Buckley, S. E. Schiff, et al. “Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus.J Immunol 158, no. 2 (January 15, 1997): 998–1005.
Markert ML, Kostyu DD, Ward FE, McLaughlin TM, Watson TJ, Buckley RH, et al. Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus. J Immunol. 1997 Jan 15;158(2):998–1005.
Markert, M. L., et al. “Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus.J Immunol, vol. 158, no. 2, Jan. 1997, pp. 998–1005.
Markert ML, Kostyu DD, Ward FE, McLaughlin TM, Watson TJ, Buckley RH, Schiff SE, Ungerleider RM, Gaynor JW, Oldham KT, Mahaffey SM, Ballow M, Driscoll DA, Hale LP, Haynes BF. Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus. J Immunol. 1997 Jan 15;158(2):998–1005.

Published In

J Immunol

ISSN

0022-1767

Publication Date

January 15, 1997

Volume

158

Issue

2

Start / End Page

998 / 1005

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Thymus Gland
  • Organ Culture Techniques
  • Infant
  • Immunology
  • Humans
  • Graft Survival
  • DiGeorge Syndrome
  • Chimera
  • 3204 Immunology