Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus.

Published

Journal Article

Transplantation of cultured postnatal human thymus was performed in a patient with complete DiGeorge syndrome. Biopsy of the graft 3 mo after implantation revealed normal CD1+ thymocytes in thymic cortical epithelial regions and CD1- thymocytes in thymic medullary epithelial regions, respectively. HLA analysis of graft thymocyte and thymic microenvironment components demonstrated that developing thymocytes and thymic macrophages were recipient derived, while thymic epithelial components were of donor origin. The patient, who initially had no T cells and had profoundly defective T cell function, developed normal T cell responses to mitogens and Ags, tolerance to donor in a mixed lymphocyte reaction, and normal Ab titers after tetanus toxoid and pneumovax immunization. Thus, transplantation of cultured postnatal human thymic tissue in humans can form functional chimeric thymic tissue, and may provide a strategy to reconstitute the peripheral T cell pool in select congenital and acquired immune deficiency syndromes.

Full Text

Duke Authors

Cited Authors

  • Markert, ML; Kostyu, DD; Ward, FE; McLaughlin, TM; Watson, TJ; Buckley, RH; Schiff, SE; Ungerleider, RM; Gaynor, JW; Oldham, KT; Mahaffey, SM; Ballow, M; Driscoll, DA; Hale, LP; Haynes, BF

Published Date

  • January 1997

Published In

Volume / Issue

  • 158 / 2

Start / End Page

  • 998 - 1005

PubMed ID

  • 8993022

Pubmed Central ID

  • 8993022

Electronic International Standard Serial Number (EISSN)

  • 1550-6606

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng