Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.

Published

Journal Article

Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell growth factor and assayed for the expression and release of human T-lymphotropic retroviruses (HTLV). Retroviruses belonging to the HTLV family and collectively designated HTLV-III were isolated from a total of 48 subjects including 18 of 21 patients wih pre-AIDS, three of four clinically normal mothers of juveniles with AIDS, 26 of 72 adult and juvenile patients with AIDS, and from one of 22 normal male homosexual subjects. No HTLV-III was detected in or isolated from 115 normal heterosexual subjects. The number of HTLV-III isolates reported here underestimates the true prevalence of the virus since many specimens were received in unsatisfactory condition. Other data show that serum samples from a high proportion of AIDS patients contain antibodies to HTLV-III. That these new isolates are members of the HTLV family but differ from the previous isolates known as HTLV-I and HTLV-II is indicated by their morphological, biological, and immunological characteristics. These results and those reported elsewhere in this issue suggest that HTLV-III may be the primary cause of AIDS.

Full Text

Duke Authors

Cited Authors

  • Gallo, RC; Salahuddin, SZ; Popovic, M; Shearer, GM; Kaplan, M; Haynes, BF; Palker, TJ; Redfield, R; Oleske, J; Safai, B

Published Date

  • May 4, 1984

Published In

Volume / Issue

  • 224 / 4648

Start / End Page

  • 500 - 503

PubMed ID

  • 6200936

Pubmed Central ID

  • 6200936

International Standard Serial Number (ISSN)

  • 0036-8075

Language

  • eng

Conference Location

  • United States