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Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides.

Publication ,  Journal Article
Palker, TJ; Clark, ME; Langlois, AJ; Matthews, TJ; Weinhold, KJ; Randall, RR; Bolognesi, DP; Haynes, BF
Published in: Proc Natl Acad Sci U S A
March 1988

A synthetic peptide (SP-10-IIIB) with an amino acid sequence [Cys-Thr-Arg-Pro-Asn-Asn-Asn-Thr-Arg-Lys-Ser-Ile-Arg-Ile-Gln-Arg-Gly-Pro -Pro-Gly-(Tyr); amino acids 303-321] from the human immunodeficiency virus (HIV) isolate human T-cell lymphotropic virus type III (HTLV-III) HTLV-IIIB envelope glycoprotein gp120 was coupled to tetanus toxoid and used to raise goat antibodies to HIV gp120. Goat anti-SP-10-IIIB serum bound to the surface of HTLV-IIIB-infected CEM T cells but not to the surface of HTLV-IIIRF-infected or uninfected CEM T cells. Anti-SP-10-IIIB antibodies also selectively bound to gp120 from lysates of HTLV-IIIB cells in immunoblot assays. Twenty-one percent of sera (28 of 175) from patients seropositive for HIV contained antibodies that reacted with SP-10-IIIB in RIA. Human anti-SP-10-IIIB antibodies affinity purified from acquired immunodeficiency syndrome (AIDS) patient serum bound to HTLV-IIIB-infected cells and immunoprecipitated gp120. Goat antibodies to SP-10-IIIB neutralized HTLV-IIIB (80% neutralization titer of 1/600), inhibited HTLV-IIIB-induced syncytium formation, but did not neutralize HIV isolates HTLV-IIIRF or HTLV-IIIMN or inhibit syncytium formation with these isolates. Also, goat antiserum to an homologous synthetic peptide [SP-10-IIIRF(A), (Cys)-Arg-Lys-Ser-Ile-Thr-Lys-Gly-Pro-Gly-Arg-Val-Ile-Tyr] from gp120 of HIV isolate HTLV-IIIRF inhibited syncytium formation by HTLV-IIIRF, but did not inhibit syncytium formation by HTLV-IIIB or by HTLV-IIIMN. Thus, the amino acid sequences of SP-10-IIIB and SP-10-IIIRF(A) define homologous regions of gp120 that are important in type-specific virus neutralization. The identification of these type-specific neutralizing epitopes should facilitate the design of a polyvalent, synthetic vaccine for AIDS.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1988

Volume

85

Issue

6

Start / End Page

1932 / 1936

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Molecular Sequence Data
  • Immune Sera
  • Humans
  • HIV
  • Epitopes
  • Antibodies
  • Animals
  • Amino Acid Sequence
  • Acquired Immunodeficiency Syndrome
 

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Palker, T. J., Clark, M. E., Langlois, A. J., Matthews, T. J., Weinhold, K. J., Randall, R. R., … Haynes, B. F. (1988). Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides. Proc Natl Acad Sci U S A, 85(6), 1932–1936. https://doi.org/10.1073/pnas.85.6.1932
Palker, T. J., M. E. Clark, A. J. Langlois, T. J. Matthews, K. J. Weinhold, R. R. Randall, D. P. Bolognesi, and B. F. Haynes. “Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides.Proc Natl Acad Sci U S A 85, no. 6 (March 1988): 1932–36. https://doi.org/10.1073/pnas.85.6.1932.
Palker TJ, Clark ME, Langlois AJ, Matthews TJ, Weinhold KJ, Randall RR, et al. Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1932–6.
Palker, T. J., et al. “Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides.Proc Natl Acad Sci U S A, vol. 85, no. 6, Mar. 1988, pp. 1932–36. Pubmed, doi:10.1073/pnas.85.6.1932.
Palker TJ, Clark ME, Langlois AJ, Matthews TJ, Weinhold KJ, Randall RR, Bolognesi DP, Haynes BF. Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1932–1936.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1988

Volume

85

Issue

6

Start / End Page

1932 / 1936

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Molecular Sequence Data
  • Immune Sera
  • Humans
  • HIV
  • Epitopes
  • Antibodies
  • Animals
  • Amino Acid Sequence
  • Acquired Immunodeficiency Syndrome