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Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome.

Publication ,  Journal Article
Davis, CM; McLaughlin, TM; Watson, TJ; Buckley, RH; Schiff, SE; Hale, LP; Haynes, BF; Markert, ML
Published in: J Clin Immunol
March 1997

Complete DiGeorge syndrome is an immunodeficiency disease characterized by thymic aplasia and the absence of functioning peripheral T cells. A patient with this syndrome was transplanted with cultured postnatal human thymic tissue. Within 5 weeks of transplantation, flow cytometry, T cell receptor V beta sequence analysis, and cell function studies showed the presence of oligoclonal populations of nonfunctional clonally expanded peripheral T cells that were derived from pretransplantation T cells present in the skin. However, at 3 months posttransplantation, a biopsy of the transplanted thymus showed normal intrathymic T cell maturation of host T cells with normal TCR V beta expression on thymocytes. By 9 months postransplantation, peripheral T cell function was restored and the TCR V beta repertoire became polyclonal, coincident with the appearance of normal T cell function. These data suggest that the transplanted thymus was responsible for the establishment of a new T cell repertoire via thymopoiesis in the chimeric thymic graft.

Duke Scholars

Published In

J Clin Immunol

DOI

ISSN

0271-9142

Publication Date

March 1997

Volume

17

Issue

2

Start / End Page

167 / 175

Location

Netherlands

Related Subject Headings

  • Thymus Gland
  • Sequence Alignment
  • Receptors, Antigen, T-Cell, alpha-beta
  • Organ Transplantation
  • Molecular Sequence Data
  • Immunology
  • Humans
  • DiGeorge Syndrome
  • Base Sequence
  • 3204 Immunology
 

Citation

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MLA
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Davis, C. M., McLaughlin, T. M., Watson, T. J., Buckley, R. H., Schiff, S. E., Hale, L. P., … Markert, M. L. (1997). Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome. J Clin Immunol, 17(2), 167–175. https://doi.org/10.1023/a:1027382600143
Davis, C. M., T. M. McLaughlin, T. J. Watson, R. H. Buckley, S. E. Schiff, L. P. Hale, B. F. Haynes, and M. L. Markert. “Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome.J Clin Immunol 17, no. 2 (March 1997): 167–75. https://doi.org/10.1023/a:1027382600143.
Davis CM, McLaughlin TM, Watson TJ, Buckley RH, Schiff SE, Hale LP, et al. Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome. J Clin Immunol. 1997 Mar;17(2):167–75.
Davis, C. M., et al. “Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome.J Clin Immunol, vol. 17, no. 2, Mar. 1997, pp. 167–75. Pubmed, doi:10.1023/a:1027382600143.
Davis CM, McLaughlin TM, Watson TJ, Buckley RH, Schiff SE, Hale LP, Haynes BF, Markert ML. Normalization of the peripheral blood T cell receptor V beta repertoire after cultured postnatal human thymic transplantation in DiGeorge syndrome. J Clin Immunol. 1997 Mar;17(2):167–175.
Journal cover image

Published In

J Clin Immunol

DOI

ISSN

0271-9142

Publication Date

March 1997

Volume

17

Issue

2

Start / End Page

167 / 175

Location

Netherlands

Related Subject Headings

  • Thymus Gland
  • Sequence Alignment
  • Receptors, Antigen, T-Cell, alpha-beta
  • Organ Transplantation
  • Molecular Sequence Data
  • Immunology
  • Humans
  • DiGeorge Syndrome
  • Base Sequence
  • 3204 Immunology