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Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry.

Publication ,  Journal Article
McCachren, SS; Haynes, BF; Niedel, JE
Published in: J Clin Immunol
January 1990

Collagenase has been implicated as playing an important role in the connective tissue destruction that is a major feature of rheumatoid arthritis. Numerous cell types in the hyperplastic rheumatoid synovium are capable of synthesizing collagenase. Past studies have used predominantly synovial fibroblasts in culture as a model system for the regulation of collagenase production, but the major cellular source of the enzyme in vivo has not been determined. Using the techniques of in situ hybridization histochemistry and indirect immunofluorescence, we determined the cellular source of collagenase in frozen sections of human synovium. Collagenase mRNA production was localized to cells along the synovial lining layer in rheumatoid arthritis. These were identified as the macrophage-like Type A synovial lining cells by immunofluorescence with antibody LeuM3. Endothelial cells, fibroblasts, and T and B lymphocytes were devoid of detectable collagenase mRNA. Synovial tissue sections from patients with osteoarthritis and trauma did not contain detectable collagenase mRNA. These data identify the Type A macrophage-like synovial lining cell as the primary source of collagenase mRNA in vivo in the rheumatoid arthritis synovium and, potentially, as a major effector cell in the tissue destruction of the disease.

Duke Scholars

Published In

J Clin Immunol

DOI

ISSN

0271-9142

Publication Date

January 1990

Volume

10

Issue

1

Start / End Page

19 / 27

Location

Netherlands

Related Subject Headings

  • Synovial Membrane
  • RNA, Messenger
  • RNA Probes
  • Nucleic Acid Hybridization
  • Microbial Collagenase
  • Macrophages
  • Immunology
  • Humans
  • Fluorescent Antibody Technique
  • Arthritis, Rheumatoid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McCachren, S. S., Haynes, B. F., & Niedel, J. E. (1990). Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry. J Clin Immunol, 10(1), 19–27. https://doi.org/10.1007/BF00917494
McCachren, S. S., B. F. Haynes, and J. E. Niedel. “Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry.J Clin Immunol 10, no. 1 (January 1990): 19–27. https://doi.org/10.1007/BF00917494.
McCachren SS, Haynes BF, Niedel JE. Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry. J Clin Immunol. 1990 Jan;10(1):19–27.
McCachren, S. S., et al. “Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry.J Clin Immunol, vol. 10, no. 1, Jan. 1990, pp. 19–27. Pubmed, doi:10.1007/BF00917494.
McCachren SS, Haynes BF, Niedel JE. Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry. J Clin Immunol. 1990 Jan;10(1):19–27.
Journal cover image

Published In

J Clin Immunol

DOI

ISSN

0271-9142

Publication Date

January 1990

Volume

10

Issue

1

Start / End Page

19 / 27

Location

Netherlands

Related Subject Headings

  • Synovial Membrane
  • RNA, Messenger
  • RNA Probes
  • Nucleic Acid Hybridization
  • Microbial Collagenase
  • Macrophages
  • Immunology
  • Humans
  • Fluorescent Antibody Technique
  • Arthritis, Rheumatoid