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Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state.

Publication ,  Journal Article
Patterson, JA; Eisinger, M; Haynes, BF; Berger, CL; Edelson, RL
Published in: J Invest Dermatol
September 1984

To establish a method for separating different keratinocyte subpopulations in the epidermis, we studied the specificity of monoclonal antibody 4F2 for keratinocytes. Preliminary screening experiments had previously demonstrated 4F2 reactivity with the epidermis. 4F2 reacted with a subpopulation (19.29 +/- 5.23%) of human epidermal cells in suspension. The membrane antigen identified by 4F2 continues to be expressed by cultured keratinocytes. In frozen tissue section using an indirect immunofluorescence technique, the 4F2-positive cells in the basal layer are sharply demarcated from the negative suprabasilar layers. Even in the hyperproliferative state of psoriasis, the 4F2 reactivity is confined to the basal layer. Cell suspensions of psoriatic epidermis demonstrated a greater percentage of reactivity with 4F2 (49.51% +/- 6.50%), probably reflecting the expanded population of basal layer cells. Monoclonal 4F2, therefore, reacts with a membrane antigen present on basal keratinocytes, and provides a probe for use in the isolation of the basal keratinocyte subpopulation. Thus, this antibody should be useful in studies of normal and aberrant differentiation of the epidermis.

Duke Scholars

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

September 1984

Volume

83

Issue

3

Start / End Page

210 / 213

Location

United States

Related Subject Headings

  • Psoriasis
  • Keratins
  • Humans
  • Fluorescent Antibody Technique
  • Epidermis
  • Dermatology & Venereal Diseases
  • Cell Division
  • Antigens, Surface
  • Antibodies, Monoclonal
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Patterson, J. A., Eisinger, M., Haynes, B. F., Berger, C. L., & Edelson, R. L. (1984). Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state. J Invest Dermatol, 83(3), 210–213. https://doi.org/10.1111/1523-1747.ep12263581
Patterson, J. A., M. Eisinger, B. F. Haynes, C. L. Berger, and R. L. Edelson. “Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state.J Invest Dermatol 83, no. 3 (September 1984): 210–13. https://doi.org/10.1111/1523-1747.ep12263581.
Patterson JA, Eisinger M, Haynes BF, Berger CL, Edelson RL. Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state. J Invest Dermatol. 1984 Sep;83(3):210–3.
Patterson, J. A., et al. “Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state.J Invest Dermatol, vol. 83, no. 3, Sept. 1984, pp. 210–13. Pubmed, doi:10.1111/1523-1747.ep12263581.
Patterson JA, Eisinger M, Haynes BF, Berger CL, Edelson RL. Monoclonal antibody 4F2 reactive with basal layer keratinocytes: studies in the normal and a hyperproliferative state. J Invest Dermatol. 1984 Sep;83(3):210–213.
Journal cover image

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

September 1984

Volume

83

Issue

3

Start / End Page

210 / 213

Location

United States

Related Subject Headings

  • Psoriasis
  • Keratins
  • Humans
  • Fluorescent Antibody Technique
  • Epidermis
  • Dermatology & Venereal Diseases
  • Cell Division
  • Antigens, Surface
  • Antibodies, Monoclonal
  • 3202 Clinical sciences