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Activation of human B lymphocytes. XIV. Characterization of the precursor of the pokeweed mitogen-induced anti-sheep red blood cell plaque-forming cell.

Publication ,  Journal Article
Dimitriu, A; Haynes, BF; Fauci, AS
Published in: J Immunol
August 1979

The precursor of the pokeweed mitogen (PWM)-induced anti-sheep red blood cell (SRBC) plaque-forming cell (PFC) in human peripheral blood was characterized. By a variety of purification procedures, it was demonstrated to be a lymphocyte with surface characteristics of a B cell. Furthermore, it was demonstrated to bind to sheep erythrocytes (E) and thus segregated with the E-rosetting T cells when T cell enrichment was performed by differential fractionation of E-rosetting cells. This binding of the PFC precursor to E was blocked by pretreating the lymphocyte with anti-human Ig before E rosetting, indicating that the PFC precursor specifically bound to SRBC by a surface Ig molecule with binding specificity for sheep red blood cell determinants. Hence, the precursor of the PWM-triggered anti-SRBC PFC is a B lymphocyte with surface Ig expressing specificity for SRBC.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 1979

Volume

123

Issue

2

Start / End Page

890 / 895

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Sheep
  • Rosette Formation
  • Rabbits
  • Pokeweed Mitogens
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin Fc Fragments
  • Immunoglobulin Fab Fragments
  • Humans
 

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 1979

Volume

123

Issue

2

Start / End Page

890 / 895

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Sheep
  • Rosette Formation
  • Rabbits
  • Pokeweed Mitogens
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin Fc Fragments
  • Immunoglobulin Fab Fragments
  • Humans