A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies.
Publication
, Journal Article
Eisenbarth, GS; Rankin, RB; Haynes, BF; Fauci, AS
Published in: J Immunol Methods
1980
We have developed a visual microtiter assay to detect solubilized cell-surface antigens which react with monoclonal antibodies. The assay depends on the ability of adsorbed monoclonal antibody to bind target cells to microtiter V wells, and the inhibition of binding by antigen. We have used this assay to follow a 600-fold purification of the human lymphocyte differentiation antigen 3A1 extracted from HSB-2 cells. Antigen 3A1 is a glycoprotein with a molecular weight of approximately 40,000 daltons.
Duke Scholars
Published In
J Immunol Methods
DOI
ISSN
0022-1759
Publication Date
1980
Volume
39
Issue
4
Start / End Page
387 / 392
Location
Netherlands
Related Subject Headings
- Immunology
- Electrophoresis, Polyacrylamide Gel
- Clone Cells
- Binding Sites, Antibody
- Antigens, Surface
- Antigen-Antibody Reactions
- Antibodies
- Adsorption
- 3204 Immunology
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Eisenbarth, G. S., Rankin, R. B., Haynes, B. F., & Fauci, A. S. (1980). A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies. J Immunol Methods, 39(4), 387–392. https://doi.org/10.1016/0022-1759(80)90239-2
Eisenbarth, G. S., R. B. Rankin, B. F. Haynes, and A. S. Fauci. “A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies.” J Immunol Methods 39, no. 4 (1980): 387–92. https://doi.org/10.1016/0022-1759(80)90239-2.
Eisenbarth GS, Rankin RB, Haynes BF, Fauci AS. A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies. J Immunol Methods. 1980;39(4):387–92.
Eisenbarth, G. S., et al. “A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies.” J Immunol Methods, vol. 39, no. 4, 1980, pp. 387–92. Pubmed, doi:10.1016/0022-1759(80)90239-2.
Eisenbarth GS, Rankin RB, Haynes BF, Fauci AS. A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies. J Immunol Methods. 1980;39(4):387–392.
Published In
J Immunol Methods
DOI
ISSN
0022-1759
Publication Date
1980
Volume
39
Issue
4
Start / End Page
387 / 392
Location
Netherlands
Related Subject Headings
- Immunology
- Electrophoresis, Polyacrylamide Gel
- Clone Cells
- Binding Sites, Antibody
- Antigens, Surface
- Antigen-Antibody Reactions
- Antibodies
- Adsorption
- 3204 Immunology
- 3101 Biochemistry and cell biology