A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies.

Published

Journal Article

We have developed a visual microtiter assay to detect solubilized cell-surface antigens which react with monoclonal antibodies. The assay depends on the ability of adsorbed monoclonal antibody to bind target cells to microtiter V wells, and the inhibition of binding by antigen. We have used this assay to follow a 600-fold purification of the human lymphocyte differentiation antigen 3A1 extracted from HSB-2 cells. Antigen 3A1 is a glycoprotein with a molecular weight of approximately 40,000 daltons.

Full Text

Duke Authors

Cited Authors

  • Eisenbarth, GS; Rankin, RB; Haynes, BF; Fauci, AS

Published Date

  • 1980

Published In

Volume / Issue

  • 39 / 4

Start / End Page

  • 387 - 392

PubMed ID

  • 7462655

Pubmed Central ID

  • 7462655

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(80)90239-2

Language

  • eng

Conference Location

  • Netherlands