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Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination.

Publication ,  Journal Article
Polonis, VR; Brown, BK; Rosa Borges, A; Zolla-Pazner, S; Dimitrov, DS; Zhang, M-Y; Barnett, SW; Ruprecht, RM; Scarlatti, G; Fenyö, E-M ...
Published in: Virology
June 5, 2008

In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.

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Published In

Virology

DOI

ISSN

0042-6822

Publication Date

June 5, 2008

Volume

375

Issue

2

Start / End Page

315 / 320

Location

United States

Related Subject Headings

  • Virology
  • Vaccination
  • Sensitivity and Specificity
  • Neutralization Tests
  • Luciferases
  • Leukocytes, Mononuclear
  • Humans
  • Hela Cells
  • HeLa Cells
  • HIV-1
 

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Polonis, V. R., Brown, B. K., Rosa Borges, A., Zolla-Pazner, S., Dimitrov, D. S., Zhang, M.-Y., … Michael, N. L. (2008). Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology, 375(2), 315–320. https://doi.org/10.1016/j.virol.2008.02.007
Polonis, Victoria R., Bruce K. Brown, Andrew Rosa Borges, Susan Zolla-Pazner, Dimiter S. Dimitrov, Mei-Yun Zhang, Susan W. Barnett, et al. “Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination.Virology 375, no. 2 (June 5, 2008): 315–20. https://doi.org/10.1016/j.virol.2008.02.007.
Polonis VR, Brown BK, Rosa Borges A, Zolla-Pazner S, Dimitrov DS, Zhang M-Y, et al. Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology. 2008 Jun 5;375(2):315–20.
Polonis, Victoria R., et al. “Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination.Virology, vol. 375, no. 2, June 2008, pp. 315–20. Pubmed, doi:10.1016/j.virol.2008.02.007.
Polonis VR, Brown BK, Rosa Borges A, Zolla-Pazner S, Dimitrov DS, Zhang M-Y, Barnett SW, Ruprecht RM, Scarlatti G, Fenyö E-M, Montefiori DC, McCutchan FE, Michael NL. Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology. 2008 Jun 5;375(2):315–320.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

June 5, 2008

Volume

375

Issue

2

Start / End Page

315 / 320

Location

United States

Related Subject Headings

  • Virology
  • Vaccination
  • Sensitivity and Specificity
  • Neutralization Tests
  • Luciferases
  • Leukocytes, Mononuclear
  • Humans
  • Hela Cells
  • HeLa Cells
  • HIV-1