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Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies.

Publication ,  Journal Article
Seaman, MS; Janes, H; Hawkins, N; Grandpre, LE; Devoy, C; Giri, A; Coffey, RT; Harris, L; Wood, B; Daniels, MG; Bhattacharya, T; Lapedes, A ...
Published in: J Virol
February 2010

The restricted neutralization breadth of vaccine-elicited antibodies is a major limitation of current human immunodeficiency virus-1 (HIV-1) candidate vaccines. In order to permit the efficient identification of vaccines with enhanced capacity for eliciting cross-reactive neutralizing antibodies (NAbs) and to assess the overall breadth and potency of vaccine-elicited NAb reactivity, we assembled a panel of 109 molecularly cloned HIV-1 Env pseudoviruses representing a broad range of genetic and geographic diversity. Viral isolates from all major circulating genetic subtypes were included, as were viruses derived shortly after transmission and during the early and chronic stages of infection. We assembled a panel of genetically diverse HIV-1-positive (HIV-1(+)) plasma pools to assess the neutralization sensitivities of the entire virus panel. When the viruses were rank ordered according to the average sensitivity to neutralization by the HIV-1(+) plasmas, a continuum of average sensitivity was observed. Clustering analysis of the patterns of sensitivity defined four subgroups of viruses: those having very high (tier 1A), above-average (tier 1B), moderate (tier 2), or low (tier 3) sensitivity to antibody-mediated neutralization. We also investigated potential associations between characteristics of the viral isolates (clade, stage of infection, and source of virus) and sensitivity to NAb. In particular, higher levels of NAb activity were observed when the virus and plasma pool were matched in clade. These data provide the first systematic assessment of the overall neutralization sensitivities of a genetically and geographically diverse panel of circulating HIV-1 strains. These reference viruses can facilitate the systematic characterization of NAb responses elicited by candidate vaccine immunogens.

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Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

February 2010

Volume

84

Issue

3

Start / End Page

1439 / 1452

Location

United States

Related Subject Headings

  • Virology
  • Humans
  • HIV-1
  • HIV Infections
  • Gene Products, env
  • Cell Line
  • Antibodies, Neutralizing
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
 

Citation

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Seaman, M. S., Janes, H., Hawkins, N., Grandpre, L. E., Devoy, C., Giri, A., … Mascola, J. R. (2010). Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies. J Virol, 84(3), 1439–1452. https://doi.org/10.1128/JVI.02108-09
Seaman, Michael S., Holly Janes, Natalie Hawkins, Lauren E. Grandpre, Colleen Devoy, Ayush Giri, Rory T. Coffey, et al. “Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies.J Virol 84, no. 3 (February 2010): 1439–52. https://doi.org/10.1128/JVI.02108-09.
Seaman MS, Janes H, Hawkins N, Grandpre LE, Devoy C, Giri A, et al. Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies. J Virol. 2010 Feb;84(3):1439–52.
Seaman, Michael S., et al. “Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies.J Virol, vol. 84, no. 3, Feb. 2010, pp. 1439–52. Pubmed, doi:10.1128/JVI.02108-09.
Seaman MS, Janes H, Hawkins N, Grandpre LE, Devoy C, Giri A, Coffey RT, Harris L, Wood B, Daniels MG, Bhattacharya T, Lapedes A, Polonis VR, McCutchan FE, Gilbert PB, Self SG, Korber BT, Montefiori DC, Mascola JR. Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies. J Virol. 2010 Feb;84(3):1439–1452.

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

February 2010

Volume

84

Issue

3

Start / End Page

1439 / 1452

Location

United States

Related Subject Headings

  • Virology
  • Humans
  • HIV-1
  • HIV Infections
  • Gene Products, env
  • Cell Line
  • Antibodies, Neutralizing
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences