Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers.

Published

Journal Article

An optimally effective AIDS vaccine would likely require the induction of both neutralizing antibody and cell-mediated immune responses, which has proven difficult to obtain in previous clinical trials. Here we report on the induction of Human Immunodeficiency Virus Type-1 (HIV-1)-specific immune responses in healthy adult volunteers that received the multi-gene, polyvalent, DNA prime-protein boost HIV-1 vaccine formulation, DP6-001, in a Phase I clinical trial conducted in healthy adult volunteers of both genders. Robust cross-subtype HIV-1-specific T cell responses were detected in IFNgamma ELISPOT assays. Furthermore, we detected high titer serum antibody responses that recognized a wide range of primary HIV-1 Env antigens and also neutralized pseudotyped viruses that express the primary Env antigens from multiple HIV-1 subtypes. These findings demonstrate that the DNA prime-protein boost approach is an effective immunization method to elicit both humoral and cell-mediated immune responses in humans, and that a polyvalent Env formulation could generate broad immune responses against HIV-1 viruses with diverse genetic backgrounds.

Full Text

Duke Authors

Cited Authors

  • Wang, S; Kennedy, JS; West, K; Montefiori, DC; Coley, S; Lawrence, J; Shen, S; Green, S; Rothman, AL; Ennis, FA; Arthos, J; Pal, R; Markham, P; Lu, S

Published Date

  • February 20, 2008

Published In

Volume / Issue

  • 26 / 8

Start / End Page

  • 1098 - 1110

PubMed ID

  • 18243434

Pubmed Central ID

  • 18243434

International Standard Serial Number (ISSN)

  • 0264-410X

Digital Object Identifier (DOI)

  • 10.1016/j.vaccine.2007.12.024

Language

  • eng

Conference Location

  • Netherlands