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Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV.

Publication ,  Journal Article
Pahar, B; Cantu, MA; Zhao, W; Kuroda, MJ; Veazey, RS; Montefiori, DC; Clements, JD; Aye, PP; Lackner, AA; Lovgren-Bengtsson, K; Sestak, K
Published in: Vaccine
November 17, 2006

The difficulty in developing an effective vaccine to contain the HIV/AIDS epidemic coupled with the fact that primary HIV-1 infection typically occurs via mucosal sites has increased emphasis on vaccine approaches that protect at mucosal surfaces. In this study we employed HIV and simian-HIV (SHIV)-derived T helper (Th) and cytotoxic T lymphocyte (CTL) single epitopes incorporated into immuno-stimulating complexes (ISCOM) as a candidate immunogens. Immunized rhesus macaques (Macaca mulatta) were challenged with CCR5-tropic SHIV(SF162p4). On the day of challenge, low levels of virus-neutralizing antibodies (Ab) and CTLs were detected in ISCOM-immunized macaques. Greater than 10(5) viral RNA copies per ml of plasma in 2/5 immunized and 3/4 control macaques were detected within 3 weeks post-challenge. Depletion of CD4+ T cells from gut-associated lymphoid tissues (GALT) was observed by post-challenge day (PCD) 14 in all macaques regardless immunization. Nonetheless, lower viral loads and relatively better preservation of peripheral CD4+ T cells following the SHIV infection was observed in ISCOM-immunized macaques. We predict that if coadministered with additional epitopes and/or more efficacious mucosal delivery system or route, HIV/SIV-derived peptide vaccines may have potential to elicit heterologous protection.

Duke Scholars

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

November 17, 2006

Volume

24

Issue

47-48

Start / End Page

6839 / 6849

Location

Netherlands

Related Subject Headings

  • fas Receptor
  • Virology
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Phenotype
  • Neutralization Tests
  • Macaca mulatta
  • Ki-67 Antigen
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pahar, B., Cantu, M. A., Zhao, W., Kuroda, M. J., Veazey, R. S., Montefiori, D. C., … Sestak, K. (2006). Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV. Vaccine, 24(47–48), 6839–6849. https://doi.org/10.1016/j.vaccine.2006.06.050
Pahar, Bapi, Mayra A. Cantu, Wei Zhao, Marcelo J. Kuroda, Ronald S. Veazey, David C. Montefiori, John D. Clements, et al. “Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV.Vaccine 24, no. 47–48 (November 17, 2006): 6839–49. https://doi.org/10.1016/j.vaccine.2006.06.050.
Pahar B, Cantu MA, Zhao W, Kuroda MJ, Veazey RS, Montefiori DC, et al. Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV. Vaccine. 2006 Nov 17;24(47–48):6839–49.
Pahar, Bapi, et al. “Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV.Vaccine, vol. 24, no. 47–48, Nov. 2006, pp. 6839–49. Pubmed, doi:10.1016/j.vaccine.2006.06.050.
Pahar B, Cantu MA, Zhao W, Kuroda MJ, Veazey RS, Montefiori DC, Clements JD, Aye PP, Lackner AA, Lovgren-Bengtsson K, Sestak K. Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV. Vaccine. 2006 Nov 17;24(47–48):6839–6849.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

November 17, 2006

Volume

24

Issue

47-48

Start / End Page

6839 / 6849

Location

Netherlands

Related Subject Headings

  • fas Receptor
  • Virology
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Phenotype
  • Neutralization Tests
  • Macaca mulatta
  • Ki-67 Antigen