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Signature for long-term vaccine-mediated control of a Simian and human immunodeficiency virus 89.6P challenge: stable low-breadth and low-frequency T-cell response capable of coproducing gamma interferon and interleukin-2.

Publication ,  Journal Article
Sadagopal, S; Amara, RR; Montefiori, DC; Wyatt, LS; Staprans, SI; Kozyr, NL; McClure, HM; Moss, B; Robinson, HL
Published in: J Virol
March 2005

In 2001, we reported 20 weeks of control of challenge with the virulent 89.6P chimera of simian and human immunodeficiency viruses (SHIV-89.6P) by a Gag-Pol-Env vaccine consisting of DNA priming and modified vaccinia virus Ankara boosting. Here we report that 22 out of 23 of these animals successfully controlled their viremia until their time of euthanasia at 200 weeks postchallenge. At euthanasia, all animals had low to undetectable viral loads and normal CD4 counts. During the long period of viral control, gamma interferon (IFN-gamma)-producing antiviral T cells were present at unexpectedly low breadths and frequencies. Most animals recognized two CD8 and one CD4 epitope and had frequencies of IFN-gamma-responding T cells from 0.01 to 0.3% of total CD8 or CD4 T cells. T-cell responses were remarkably stable over time and, unlike responses in most immunodeficiency virus infections, maintained good functional characteristics, as evidenced by coproduction of IFN-gamma and interleukin-2. Overall, high titers of binding and neutralizing antibody persisted throughout the postchallenge period. Encouragingly, long-term control was effective in macaques of diverse histocompatibility types.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

March 2005

Volume

79

Issue

6

Start / End Page

3243 / 3253

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Viral Load
  • Vaccinia virus
  • Vaccines, DNA
  • T-Lymphocytes
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • RNA, Viral
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sadagopal, S., Amara, R. R., Montefiori, D. C., Wyatt, L. S., Staprans, S. I., Kozyr, N. L., … Robinson, H. L. (2005). Signature for long-term vaccine-mediated control of a Simian and human immunodeficiency virus 89.6P challenge: stable low-breadth and low-frequency T-cell response capable of coproducing gamma interferon and interleukin-2. J Virol, 79(6), 3243–3253. https://doi.org/10.1128/JVI.79.6.3243-3253.2005
Sadagopal, Shanmugalakshmi, Rama Rao Amara, David C. Montefiori, Linda S. Wyatt, Silvija I. Staprans, Natalia L. Kozyr, Harold M. McClure, Bernard Moss, and Harriet L. Robinson. “Signature for long-term vaccine-mediated control of a Simian and human immunodeficiency virus 89.6P challenge: stable low-breadth and low-frequency T-cell response capable of coproducing gamma interferon and interleukin-2.J Virol 79, no. 6 (March 2005): 3243–53. https://doi.org/10.1128/JVI.79.6.3243-3253.2005.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

March 2005

Volume

79

Issue

6

Start / End Page

3243 / 3253

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Viral Load
  • Vaccinia virus
  • Vaccines, DNA
  • T-Lymphocytes
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • RNA, Viral