Immunogenicity in macaques of the clinical product for a clade B DNA/MVA HIV vaccine: elicitation of IFN-gamma, IL-2, and TNF-alpha coproducing CD4 and CD8 T cells.

Published

Journal Article

The clinical product for a clade B HIV DNA/MVA vaccine expressing Gag, Pol, and Env has been tested for immunogenicity in macaques. Responding T cells were at the threshold for detection following DNA priming at weeks 0 and 8 but underwent sharp expansions and contractions following MVA boosting at weeks 16 and 24. Both CD4 and CD8 T cell responses had high frequencies of cytokine coproducing cells with >50% of the memory cells coproducing multiple cytokines including IL-2. The highest responses were elicited to Gag, followed by Env and then Pol. In two of six macaques, the vaccine also elicited low levels of neutralizing Ab for easy to neutralize clade B isolates.

Full Text

Duke Authors

Cited Authors

  • Robinson, HL; Sharma, S; Zhao, J; Kannanganat, S; Lai, L; Chennareddi, L; Yu, T; Montefiori, DC; Amara, RR; Wyatt, LS; Moss, B

Published Date

  • December 2007

Published In

Volume / Issue

  • 23 / 12

Start / End Page

  • 1555 - 1562

PubMed ID

  • 18160013

Pubmed Central ID

  • 18160013

Electronic International Standard Serial Number (EISSN)

  • 1931-8405

International Standard Serial Number (ISSN)

  • 0889-2229

Digital Object Identifier (DOI)

  • 10.1089/aid.2007.0165

Language

  • eng