Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1.
Publication
, Journal Article
Platt, EJ; Bilska, M; Kozak, SL; Kabat, D; Montefiori, DC
Published in: J Virol
August 2009
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
Duke Scholars
Published In
J Virol
DOI
EISSN
1098-5514
Publication Date
August 2009
Volume
83
Issue
16
Start / End Page
8289 / 8292
Location
United States
Related Subject Headings
- Virology
- Specimen Handling
- Neutralization Tests
- Leukemia Virus, Murine
- Humans
- HIV-1
- HIV Infections
- Cell Line
- 32 Biomedical and clinical sciences
- 31 Biological sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Platt, E. J., Bilska, M., Kozak, S. L., Kabat, D., & Montefiori, D. C. (2009). Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. J Virol, 83(16), 8289–8292. https://doi.org/10.1128/JVI.00709-09
Platt, Emily J., Miroslawa Bilska, Susan L. Kozak, David Kabat, and David C. Montefiori. “Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1.” J Virol 83, no. 16 (August 2009): 8289–92. https://doi.org/10.1128/JVI.00709-09.
Platt EJ, Bilska M, Kozak SL, Kabat D, Montefiori DC. Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. J Virol. 2009 Aug;83(16):8289–92.
Platt, Emily J., et al. “Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1.” J Virol, vol. 83, no. 16, Aug. 2009, pp. 8289–92. Pubmed, doi:10.1128/JVI.00709-09.
Platt EJ, Bilska M, Kozak SL, Kabat D, Montefiori DC. Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. J Virol. 2009 Aug;83(16):8289–8292.
Published In
J Virol
DOI
EISSN
1098-5514
Publication Date
August 2009
Volume
83
Issue
16
Start / End Page
8289 / 8292
Location
United States
Related Subject Headings
- Virology
- Specimen Handling
- Neutralization Tests
- Leukemia Virus, Murine
- Humans
- HIV-1
- HIV Infections
- Cell Line
- 32 Biomedical and clinical sciences
- 31 Biological sciences