Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1.
Journal Article (Journal Article)
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
Full Text
Duke Authors
Cited Authors
- Platt, EJ; Bilska, M; Kozak, SL; Kabat, D; Montefiori, DC
Published Date
- August 2009
Published In
Volume / Issue
- 83 / 16
Start / End Page
- 8289 - 8292
PubMed ID
- 19474095
Pubmed Central ID
- 19474095
Electronic International Standard Serial Number (EISSN)
- 1098-5514
Digital Object Identifier (DOI)
- 10.1128/JVI.00709-09
Language
- eng
Conference Location
- United States