Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.

Published

Journal Article

The HIV-1 gp120 V3 loop is a potent inducer of neutralizing antibodies for T cell line adapted-HIV-1, but less so for primary isolates. We hypothesized that peptides representative of the diversity of natural HIV-1 V3 loop variants might capture elements of conserved higher order structures and so stimulate broadly reactive neutralizing antibodies. We designed a panel of 29 subtype B V3 sequences postulated to reflect the range of V3 diversity. These peptides were used to immunize guinea pigs. The most effective peptide (62.19) clustered around the subtype B consensus sequence and induced antibodies that reproducibly neutralized 31% of the subtype B HIV-1 primary isolates evaluated, but exhibited limited cross-neutralization of non-subtype B HIV-1 strains. Taken together, these data demonstrated that the limited neutralization profile of antibodies induced by optimal subtype B V3 motifs likely represents the maximum breadth of neutralization of subtype B HIV-1 primary isolates attainable by anti-V3 peptide antibodies.

Full Text

Duke Authors

Cited Authors

  • Haynes, BF; Ma, B; Montefiori, DC; Wrin, T; Petropoulos, CJ; Sutherland, LL; Scearce, RM; Denton, C; Xia, S-M; Korber, BT; Liao, H-X

Published Date

  • February 5, 2006

Published In

Volume / Issue

  • 345 / 1

Start / End Page

  • 44 - 55

PubMed ID

  • 16242749

Pubmed Central ID

  • 16242749

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2005.08.042

Language

  • eng

Conference Location

  • United States