Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660.

Published

Journal Article

VEE replicon particles (VRP), non-propagating vaccine vectors derived from Venezuelan equine encephalitis virus (VEE), were engineered to express immunogens from the cloned isolate SIVsmH-4, combined in a vaccine cocktail and inoculated subcutaneously to immunize rhesus macaques. The virulent, uncloned challenge stock, SIVsmE660, represented a type of heterologous challenge and the intrarectal challenge modeled infection across a mucosal surface. Prechallenge neutralizing antibodies against SIVsmH-4 were induced in all vaccinates, and a prechallenge cellular immune response could be detected in one of six. Post-challenge, virus loads were reduced at the peak, at set point and at termination (41 weeks post-challenge), although these differences did not reach statistical significance. Significantly elevated levels of CD4+ T cells were observed post-challenge. A strong correlation was noted between a net increase in CD4+ T cell count and lowered virus load at set point.

Full Text

Duke Authors

Cited Authors

  • Johnston, RE; Johnson, PR; Connell, MJ; Montefiori, DC; West, A; Collier, ML; Cecil, C; Swanstrom, R; Frelinger, JA; Davis, NL

Published Date

  • October 10, 2005

Published In

Volume / Issue

  • 23 / 42

Start / End Page

  • 4969 - 4979

PubMed ID

  • 16005121

Pubmed Central ID

  • 16005121

International Standard Serial Number (ISSN)

  • 0264-410X

Digital Object Identifier (DOI)

  • 10.1016/j.vaccine.2005.05.034

Language

  • eng

Conference Location

  • Netherlands