Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660.
Journal Article (Journal Article)
VEE replicon particles (VRP), non-propagating vaccine vectors derived from Venezuelan equine encephalitis virus (VEE), were engineered to express immunogens from the cloned isolate SIVsmH-4, combined in a vaccine cocktail and inoculated subcutaneously to immunize rhesus macaques. The virulent, uncloned challenge stock, SIVsmE660, represented a type of heterologous challenge and the intrarectal challenge modeled infection across a mucosal surface. Prechallenge neutralizing antibodies against SIVsmH-4 were induced in all vaccinates, and a prechallenge cellular immune response could be detected in one of six. Post-challenge, virus loads were reduced at the peak, at set point and at termination (41 weeks post-challenge), although these differences did not reach statistical significance. Significantly elevated levels of CD4+ T cells were observed post-challenge. A strong correlation was noted between a net increase in CD4+ T cell count and lowered virus load at set point.
Full Text
Duke Authors
Cited Authors
- Johnston, RE; Johnson, PR; Connell, MJ; Montefiori, DC; West, A; Collier, ML; Cecil, C; Swanstrom, R; Frelinger, JA; Davis, NL
Published Date
- October 10, 2005
Published In
Volume / Issue
- 23 / 42
Start / End Page
- 4969 - 4979
PubMed ID
- 16005121
International Standard Serial Number (ISSN)
- 0264-410X
Digital Object Identifier (DOI)
- 10.1016/j.vaccine.2005.05.034
Language
- eng
Conference Location
- Netherlands