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Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production.

Publication ,  Journal Article
Geonnotti, AR; Bilska, M; Yuan, X; Ochsenbauer, C; Edmonds, TG; Kappes, JC; Liao, H-X; Haynes, BF; Montefiori, DC
Published in: AIDS Res Hum Retroviruses
March 2010

Bacterial lipopolysaccharide (endotoxin) is a frequent contaminant of biological specimens and is also known to be a potent inducer of beta-chemokines and other soluble factors that inhibit HIV-1 infection in vitro. Though lipopolysaccharide (LPS) has been shown to stimulate the production of soluble HIV-1 inhibitors in cultures of monocyte-derived macrophages, the ability of LPS to induce similar inhibitors in other cell types is poorly characterized. Here we show that LPS exhibits potent anti-HIV activity in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs) but has no detectable anti-HIV-1 activity in TZM-bl cells. The anti-HIV-1 activity of LPS in PBMCs was strongly associated with the production of beta-chemokines from CD14-positive monocytes. Culture supernatants from LPS-stimulated PBMCs exhibited potent anti-HIV-1 activity when added to TZM-bl cells but, in this case, the antiviral activity appeared to be related to IFN-gamma rather than to beta-chemokines. These observations indicate that LPS stimulates PBMCs to produce a complex array of soluble HIV-1 inhibitors, including beta-chemokines and IFN-gamma, that differentially inhibit HIV-1 depending on the target cell type. The results also highlight the need to use endotoxin-free specimens to avoid artifacts when assessing HIV-1-specific neutralizing antibodies in PBMC-based assays.

Duke Scholars

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

March 2010

Volume

26

Issue

3

Start / End Page

279 / 291

Location

United States

Related Subject Headings

  • Virology
  • Salmonella enterica
  • Neutralization Tests
  • Lipopolysaccharides
  • Leukocytes, Mononuclear
  • Interferon-gamma
  • Humans
  • HIV-1
  • HIV Infections
  • Escherichia coli
 

Citation

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Geonnotti, A. R., Bilska, M., Yuan, X., Ochsenbauer, C., Edmonds, T. G., Kappes, J. C., … Montefiori, D. C. (2010). Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production. AIDS Res Hum Retroviruses, 26(3), 279–291. https://doi.org/10.1089/aid.2009.0186
Geonnotti, Anthony R., Miroslawa Bilska, Xing Yuan, Christina Ochsenbauer, Tara G. Edmonds, John C. Kappes, Hua-Xin Liao, Barton F. Haynes, and David C. Montefiori. “Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production.AIDS Res Hum Retroviruses 26, no. 3 (March 2010): 279–91. https://doi.org/10.1089/aid.2009.0186.
Geonnotti AR, Bilska M, Yuan X, Ochsenbauer C, Edmonds TG, Kappes JC, et al. Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production. AIDS Res Hum Retroviruses. 2010 Mar;26(3):279–91.
Geonnotti, Anthony R., et al. “Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production.AIDS Res Hum Retroviruses, vol. 26, no. 3, Mar. 2010, pp. 279–91. Pubmed, doi:10.1089/aid.2009.0186.
Geonnotti AR, Bilska M, Yuan X, Ochsenbauer C, Edmonds TG, Kappes JC, Liao H-X, Haynes BF, Montefiori DC. Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production. AIDS Res Hum Retroviruses. 2010 Mar;26(3):279–291.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

March 2010

Volume

26

Issue

3

Start / End Page

279 / 291

Location

United States

Related Subject Headings

  • Virology
  • Salmonella enterica
  • Neutralization Tests
  • Lipopolysaccharides
  • Leukocytes, Mononuclear
  • Interferon-gamma
  • Humans
  • HIV-1
  • HIV Infections
  • Escherichia coli