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Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels.

Publication ,  Journal Article
Pal, R; Venzon, D; Santra, S; Kalyanaraman, VS; Montefiori, DC; Hocker, L; Hudacik, L; Rose, N; Nacsa, J; Edghill-Smith, Y; Moniuszko, M ...
Published in: J Virol
April 2006

Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines expressing Gag, Pol, and gp120 (vCP250) or Gag, Pol, and gp160 (vCP1420) in a prime-boost protocol with their homologous vaccine native Env proteins. The relative efficacy was measured against a high-dose mucosal exposure to the pathogenic neutralization-resistant variant SHIV(KU2) (simian-human immunodeficiency virus). Systemic immunization with both vaccine regimens decreased viral load levels not only in blood but unexpectedly also in mucosal sites and protected macaques from peripheral CD4+ T-cell loss. This protective effect was stronger when the gp120 antigen was included in the vaccine. Inclusion of recombinant Tat protein in the boosting phase along with the Env protein did not contribute further to the preservation of CD4+ T cells. Thus, systemic immunization with ALVAC-HIV-1 vaccine candidates elicits anti-HIV-1 immune responses able to contain virus replication also at mucosal sites in macaques.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 2006

Volume

80

Issue

8

Start / End Page

3732 / 3742

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Viral Load
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Research Design
  • RNA, Viral
  • Macaca mulatta
  • Immunization, Secondary
  • HIV-1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pal, R., Venzon, D., Santra, S., Kalyanaraman, V. S., Montefiori, D. C., Hocker, L., … Franchini, G. (2006). Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels. J Virol, 80(8), 3732–3742. https://doi.org/10.1128/JVI.80.8.3732-3742.2006
Pal, Ranajit, David Venzon, Sampa Santra, Vaniambadi S. Kalyanaraman, David C. Montefiori, Lindsey Hocker, Lauren Hudacik, et al. “Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels.J Virol 80, no. 8 (April 2006): 3732–42. https://doi.org/10.1128/JVI.80.8.3732-3742.2006.
Pal R, Venzon D, Santra S, Kalyanaraman VS, Montefiori DC, Hocker L, Hudacik L, Rose N, Nacsa J, Edghill-Smith Y, Moniuszko M, Hel Z, Belyakov IM, Berzofsky JA, Parks RW, Markham PD, Letvin NL, Tartaglia J, Franchini G. Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels. J Virol. 2006 Apr;80(8):3732–3742.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 2006

Volume

80

Issue

8

Start / End Page

3732 / 3742

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Viral Load
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Research Design
  • RNA, Viral
  • Macaca mulatta
  • Immunization, Secondary
  • HIV-1