Phase 1 safety and immunogenicity testing of DNA and recombinant modified vaccinia Ankara vaccines expressing HIV-1 virus-like particles.

Published

Journal Article

Recombinant DNA and modified vaccinia virus Ankara (rMVA) vaccines represent a promising approach to an HIV/AIDS vaccine. This Phase 1 clinical trial compared the safety and immunogenicity of a rMVA vaccine administered with and without DNA vaccine primingGeoVax pGA2/JS7 DNA (D) and MVA/HIV62 (M) vaccines encode noninfectious virus-like particles. Intramuscular needle injections were used to deliver placebo, 2 doses of DNA followed by 2 doses of rMVA (DDMM), one dose of DNA followed by 2 doses of rMVA (DMM), or 3 doses of rMVA (MMM) to HIV-seronegative participants.Local and systemic symptoms were mild or moderate. Immune response rates for CD4 + and CD8 + T cells were highest in the DDMM group and lowest in the MMM group (77% vs 43% CD4 + and 42% vs 17% CD8 +). In contrast, response rates for Env binding and neutralizing Ab were highest in the MMM group. The DMM group had intermediate response rates. A 1/10th-dose DDMM regimen induced similar T cell but reduced Ab response rates compared with the full-dose DDMM.MVA62 was well tolerated and elicited different patterns of T cell and Ab responses when administered alone or in combination with the JS7 DNA vaccine.

Full Text

Duke Authors

Cited Authors

  • Goepfert, PA; Elizaga, ML; Sato, A; Qin, L; Cardinali, M; Hay, CM; Hural, J; DeRosa, SC; DeFawe, OD; Tomaras, GD; Montefiori, DC; Xu, Y; Lai, L; Kalams, SA; Baden, LR; Frey, SE; Blattner, WA; Wyatt, LS; Moss, B; Robinson, HL; National Institute of Allergy and Infectious Diseases HIV Vaccine Trials Network,

Published Date

  • March 2011

Published In

Volume / Issue

  • 203 / 5

Start / End Page

  • 610 - 619

PubMed ID

  • 21282192

Pubmed Central ID

  • 21282192

Electronic International Standard Serial Number (EISSN)

  • 1537-6613

International Standard Serial Number (ISSN)

  • 0022-1899

Digital Object Identifier (DOI)

  • 10.1093/infdis/jiq105

Language

  • eng