Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.
Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cytometry measurement of population distributions of molecular markers to be deconvoluted in terms of subpopulation-specific rate parameters distinguishing commitment to differentiation, proliferation of differentiated cells, and proliferation of undifferentiated cells (i.e., self-renewal). We validate this model-based parameter determination by means of dedicated, independent cell-tracking studies. Our approach facilitates interpretation of relationships underlying effects of external cues on cell responses in differentiating cultures via intracellular signals.
Duke Scholars
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Related Subject Headings
- Stem Cells
- Models, Biological
- Mice
- Flow Cytometry
- Extracellular Matrix Proteins
- Dose-Response Relationship, Drug
- Cytokines
- Computer Simulation
- Cells, Cultured
- Cell Separation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Stem Cells
- Models, Biological
- Mice
- Flow Cytometry
- Extracellular Matrix Proteins
- Dose-Response Relationship, Drug
- Cytokines
- Computer Simulation
- Cells, Cultured
- Cell Separation