Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.
Journal Article (Journal Article)
Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cytometry measurement of population distributions of molecular markers to be deconvoluted in terms of subpopulation-specific rate parameters distinguishing commitment to differentiation, proliferation of differentiated cells, and proliferation of undifferentiated cells (i.e., self-renewal). We validate this model-based parameter determination by means of dedicated, independent cell-tracking studies. Our approach facilitates interpretation of relationships underlying effects of external cues on cell responses in differentiating cultures via intracellular signals.
Full Text
Duke Authors
Cited Authors
- Prudhomme, WA; Duggar, KH; Lauffenburger, DA
Published Date
- November 5, 2004
Published In
Volume / Issue
- 88 / 3
Start / End Page
- 264 - 272
PubMed ID
- 15486930
International Standard Serial Number (ISSN)
- 0006-3592
Digital Object Identifier (DOI)
- 10.1002/bit.20244
Language
- eng
Conference Location
- United States