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Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.

Publication ,  Journal Article
Prudhomme, WA; Duggar, KH; Lauffenburger, DA
Published in: Biotechnol Bioeng
November 5, 2004

Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cytometry measurement of population distributions of molecular markers to be deconvoluted in terms of subpopulation-specific rate parameters distinguishing commitment to differentiation, proliferation of differentiated cells, and proliferation of undifferentiated cells (i.e., self-renewal). We validate this model-based parameter determination by means of dedicated, independent cell-tracking studies. Our approach facilitates interpretation of relationships underlying effects of external cues on cell responses in differentiating cultures via intracellular signals.

Duke Scholars

Published In

Biotechnol Bioeng

DOI

ISSN

0006-3592

Publication Date

November 5, 2004

Volume

88

Issue

3

Start / End Page

264 / 272

Location

United States

Related Subject Headings

  • Stem Cells
  • Models, Biological
  • Mice
  • Flow Cytometry
  • Extracellular Matrix Proteins
  • Dose-Response Relationship, Drug
  • Cytokines
  • Computer Simulation
  • Cells, Cultured
  • Cell Separation
 

Citation

APA
Chicago
ICMJE
MLA
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Prudhomme, W. A., Duggar, K. H., & Lauffenburger, D. A. (2004). Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix. Biotechnol Bioeng, 88(3), 264–272. https://doi.org/10.1002/bit.20244
Prudhomme, Wendy A., Keith H. Duggar, and Douglas A. Lauffenburger. “Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.Biotechnol Bioeng 88, no. 3 (November 5, 2004): 264–72. https://doi.org/10.1002/bit.20244.
Prudhomme, Wendy A., et al. “Cell population dynamics model for deconvolution of murine embryonic stem cell self-renewal and differentiation responses to cytokines and extracellular matrix.Biotechnol Bioeng, vol. 88, no. 3, Nov. 2004, pp. 264–72. Pubmed, doi:10.1002/bit.20244.
Journal cover image

Published In

Biotechnol Bioeng

DOI

ISSN

0006-3592

Publication Date

November 5, 2004

Volume

88

Issue

3

Start / End Page

264 / 272

Location

United States

Related Subject Headings

  • Stem Cells
  • Models, Biological
  • Mice
  • Flow Cytometry
  • Extracellular Matrix Proteins
  • Dose-Response Relationship, Drug
  • Cytokines
  • Computer Simulation
  • Cells, Cultured
  • Cell Separation