Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.
Journal Article (Journal Article)
The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, and count 3 separate microsphere colors in 200 microm thick tissue sections from piglet brain. In resting newborn piglets (n=6) on isoflurane anesthesia, we measured a median total cerebral blood flow (CBF) of 105 ml/min/100g (range 27-206 ml/min/100 g). Compared with other FM analysis methods, our method reduces the time required to determine blood flow, improves accuracy in lipid-rich tissues and large tissue regions and, unlike the radiolabeled microsphere method, can be combined with histological analysis.
Full Text
Duke Authors
Cited Authors
- Eucker, SA; Hoffman, BD; Natesh, R; Ralston, J; Armstead, WM; Margulies, SS
Published Date
- April 22, 2010
Published In
Volume / Issue
- 1326 /
Start / End Page
- 128 - 134
PubMed ID
- 20193669
Pubmed Central ID
- PMC2855885
Electronic International Standard Serial Number (EISSN)
- 1872-6240
Digital Object Identifier (DOI)
- 10.1016/j.brainres.2010.02.059
Language
- eng
Conference Location
- Netherlands