Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.

Journal Article (Journal Article)

The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, and count 3 separate microsphere colors in 200 microm thick tissue sections from piglet brain. In resting newborn piglets (n=6) on isoflurane anesthesia, we measured a median total cerebral blood flow (CBF) of 105 ml/min/100g (range 27-206 ml/min/100 g). Compared with other FM analysis methods, our method reduces the time required to determine blood flow, improves accuracy in lipid-rich tissues and large tissue regions and, unlike the radiolabeled microsphere method, can be combined with histological analysis.

Full Text

Duke Authors

Cited Authors

  • Eucker, SA; Hoffman, BD; Natesh, R; Ralston, J; Armstead, WM; Margulies, SS

Published Date

  • April 22, 2010

Published In

Volume / Issue

  • 1326 /

Start / End Page

  • 128 - 134

PubMed ID

  • 20193669

Pubmed Central ID

  • PMC2855885

Electronic International Standard Serial Number (EISSN)

  • 1872-6240

Digital Object Identifier (DOI)

  • 10.1016/j.brainres.2010.02.059


  • eng

Conference Location

  • Netherlands